Anti-SDHB Rabbit Monoclonal Antibody | MyBioSource MBS179550 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-SDHB Rabbit Monoclonal Antibody

catalog :

MBS179550

quantity :

0.03 mg

price :

160 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

[ABDF-19]

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of SDHB expression in A431 cell lysate (MBS179550). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDHB monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SDHB

product information

catalog number :

MBS179550

products type :

Antibody

products full name :

Anti-SDHB Rabbit Monoclonal Antibody

products short name :

[SDHB]

products name syn :

[Succinate dehydrogenase [ubiquinone] iron-sulfur subunit; mitochondrial; 1.3.5.1; Iron-sulfur subunit of complex II; Ip; SDHB; SDH; SDH1]

other names :

[succinate dehydrogenase; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; succinate dehydrogenase complex iron sulfur subunit B; Iron-sulfur subunit of complex II; Ip]

products gene name :

[SDHB]

other gene names :

[SDHB; SDHB; IP; SDH; CWS2; PGL4; SDH1; SDH2; SDHIP; SDH; SDH1; Ip]

clonality :

Monoclonal

clone :

[ABDF-19]

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

280

purity :

Affinity-chromatography

form :

Liquid

storage stability :

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

tested application :

Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)

app notes :

WB: 1:500-1:2000. IHC: 1:50-1:200. IP: 1:50. FC/FACS: 1:50. Anti-SDHB Rabbit Monoclonal Antibody tested for Flow Cytometry (FC), Immunoprecipitation (IP), Immunohistochemistry (IHC), and Western Blot (WB) in Human, Mouse, and Rat.

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human SDHB. Subcellular Localization: Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.

other info2 :

Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Conjugate: No

products categories :

Signal Transduction; Metabolism; Mitochondrial; Cell Biology; Other Antibodies; Oxidative Atress; Cancer; Cancer Metabolism; Metabolic Signaling Pathway; Metabolism of Carbohydrates; Metabolism; Pathways and Processes; Mitochondrial Metabolism; Mitochondr

products description :

Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).

ncbi gi num :

115387094

ncbi acc num :

NP_002991.2

ncbi gb acc num :

NM_003000.2

uniprot acc num :

P21912

ncbi mol weight :

31630 MW

ncbi pathways :

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Citrate Cycle (TCA Cycle) Pathway (82927); Citrate Cycle (TCA Cycle) Pathway (288); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (855811); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (468202); Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate = Oxaloacetate Pathway (413348); Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate = Oxaloacetate Pathway (468204)

ncbi summary :

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq, Jul 2008]

uniprot summary :

size1 :

0.03 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

340

size3 :

0.1 mg + Biotin Secondary (MBS176645)

price3 :

340

size4 :

0.1 mg + HRP Secondary (MBS176703)

price4 :

340

more info or order :

MyBioSource product webpage