Anti-IRF5 Rabbit Monoclonal Antibody | MyBioSource MBS179503 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-IRF5 Rabbit Monoclonal Antibody

catalog :

MBS179503

quantity :

0.03 mg

price :

160 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of IRF5 expression in THP-1 cell lysate (MBS179503). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IRF5

product information

catalog number :

MBS179503

products type :

Antibody

products full name :

Anti-IRF5 Rabbit Monoclonal Antibody

products short name :

[IRF5]

products name syn :

[Interferon regulatory factor 5; IRF-5; IRF5]

other names :

[interferon regulatory factor 5 isoform b; Interferon regulatory factor 5; interferon regulatory factor 5; interferon regulatory factor 5]

products gene name :

[IRF5]

other gene names :

[IRF5; IRF5; SLEB10; IRF-5]

clonality :

Monoclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

147

purity :

Affinity-chromatography

form :

Liquid

storage stability :

Store at -20°C for one year. After reconstitution, at 4° C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing. Ships in Dry Ice.

tested application :

Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

app notes :

WB: 1:500-1:1000. IHC: 1:50-1:200. ICC/IF: 1:100-1:500. IP: 1:100. FC/FACS: 1:50

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human IRF5. Subcellular Localization: Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.

other info2 :

Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Conjugate: No

products categories :

Immunology; Innate Immunity; Cytokines; interferons; Epigenetics and Nuclear Signaling; Transcription; polymerase associated factors; pol iii Transcription; Domain Families; HLH/Leucine Zipper; helix-turn-helix; Transcription Factors

products description :

Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling.

ncbi gi num :

291190719

ncbi acc num :

NP_001092097.2

ncbi gb acc num :

NM_001098627.3

uniprot acc num :

Q13568

ncbi mol weight :

56044 MW

ncbi pathways :

Apoptosis Pathway (198797); Cytokine Signaling In Immune System Pathway (366171); Direct P53 Effectors Pathway (137939); Immune System Pathway (106386); Interferon Signaling Pathway (187103); Interferon Alpha/beta Signaling Pathway (187104); Interferon Gamma Signaling Pathway (187106); Regulation Of Toll-like Receptor Signaling Pathway (920955); Senescence And Autophagy Pathway (198780); Toll-like Receptor Signaling Pathway (83076)

ncbi summary :

This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq, Dec 2016]

uniprot summary :

Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling.

size1 :

0.03 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

340

size3 :

0.1 mg + Biotin Secondary (MBS176645)

price3 :

340

size4 :

0.1 mg + HRP Secondary (MBS176703)

price4 :

340

more info or order :

MyBioSource product webpage