Anti-GATA3 Rabbit Monoclonal Antibody | MyBioSource MBS179382 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-GATA3 Rabbit Monoclonal Antibody

catalog :

MBS179382

quantity :

0.03 mg

price :

160 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

[ABBH-7]

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS, chromatin immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of GATA3 expression in (1) SH-SY5Y cell lysate; (2) RAW 2647 cell lysate (MBS179382). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GATA3

image 2 :

HC analysis of GATA3 using anti-GATA3 antibody (MBS179382) on human bladder cancer. GATA3 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GATA3 Antibody (MBS179382) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(MBS176451) with DAB as the chromogen.

product information

catalog number :

MBS179382

products type :

Antibody

products full name :

Anti-GATA3 Rabbit Monoclonal Antibody

products short name :

[GATA3]

products name syn :

[Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3; GATA3]

other names :

[trans-acting T-cell-specific transcription factor GATA-3 isoform 1; Trans-acting T-cell-specific transcription factor GATA-3; trans-acting T-cell-specific transcription factor GATA-3; GATA binding protein 3; GATA-binding factor 3]

products gene name :

[GATA3]

other gene names :

[GATA3; GATA3; HDR; HDRS]

clonality :

Monoclonal

clone :

[ABBH-7]

host :

Rabbit

reactivity :

Human

purity :

Affinity-chromatography

concentration :

0.5-1 mg/ml, actual concentration vary by lot. Use suggested dilution ration to decide dilution procedure.

storage stability :

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

tested application :

Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

app notes :

WB 1:500-1:2000. IHC 1:50-1:200. ICC/IF 1:50-1:200. Flow Cytometry 1:100. ChIP

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Form: Liquid. Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Immunogen: A synthesized peptide derived from human GATA3.

products categories :

Immunology; Adaptive Immunity; T Cells; NON-CD; Epigenetics and Nuclear Signaling; Transcription; Domain Families; zinc finger; Stem Cells; Lineage Markers; Ectoderm; ChIP'ing Antibodies; Developmental Biology; Lineage Specification

products description :

Anti-GATA3 Rabbit Monoclonal Antibody tested for ChIP, Flow Cytometry, IF, IHC, ICC, WB in Human

ncbi gi num :

50541959

ncbi acc num :

NP_001002295.1

ncbi gb acc num :

NM_001002295.1

uniprot acc num :

P23771

ncbi pathways :

Adipogenesis Pathway (198832); C-MYB Transcription Factor Network Pathway (138073); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196); Glucocorticoid Receptor Regulatory Network Pathway (138014); Hemostasis Pathway (106028); IL27-mediated Signaling Events Pathway (137929); Notch Signaling Pathway (169345); Regulation Of Nuclear SMAD2/3 Signaling Pathway (137963); SIDS Susceptibility Pathways (198901)

ncbi summary :

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]

uniprot summary :

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.

size1 :

0.03 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

340

size3 :

0.1 mg + Biotin Secondary (MBS176645)

price3 :

340

size4 :

0.1 mg + HRP Secondary (MBS176703)

price4 :

340

more info or order :

MyBioSource product webpage