Anti-MIF Rabbit Monoclonal Antibody | MyBioSource MBS179316 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-MIF Rabbit Monoclonal Antibody

catalog :

MBS179316

quantity :

0.03 mg

price :

160 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

[ABAG-13]

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of MIF expression in (1) THP-1 cell lysate; (2) Mouse brain lysate (MBS179316). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIF monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MIF

product information

catalog number :

MBS179316

products type :

Antibody

products full name :

Anti-MIF Rabbit Monoclonal Antibody

products short name :

[MIF]

products name syn :

[Macrophage migration inhibitory factor; MIF; 5.3.2.1; Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase; 5.3.3.12; Phenylpyruvate tautomerase; MIF; GLIF; MMIF]

other names :

[macrophage migration inhibitory factor; Macrophage migration inhibitory factor; macrophage migration inhibitory factor; macrophage migration inhibitory factor; Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase (EC:5.3.3.12); Phenylpyruvate tautomerase]

products gene name :

[MIF]

other gene names :

[MIF; MIF; GIF; GLIF; MMIF; GLIF; MMIF; MIF; GIF]

clonality :

Monoclonal

isotype :

IgG

clone :

[ABAG-13]

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

115

purity :

Affinity-chromatography

form :

Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

storage stability :

Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

tested application :

Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)

app notes :

Suggested Dilutions: . WB: 1:500-1:2000. IP: 1:50. FC/FACS: 1:50

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human MIF

other info2 :

Conjugate: No

products categories :

Immunology; Innate Immunity; macrophage/inflamm.

ncbi gi num :

4505185

ncbi acc num :

NP_002406.1

ncbi gb acc num :

NM_002415.1

uniprot acc num :

P14174

ncbi pathways :

Adipogenesis Pathway (198832); Phenylalanine Metabolism Pathway (82960); Phenylalanine Metabolism Pathway (327); Spinal Cord Injury Pathway (739007); Tyrosine Metabolism Pathway (82959); Tyrosine Metabolism Pathway (325)

ncbi summary :

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]

uniprot summary :

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

size1 :

0.03 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

340

size3 :

0.1 mg + Biotin Secondary (MBS176645)

price3 :

340

size4 :

0.1 mg + HRP Secondary (MBS176703)

price4 :

340

more info or order :

MyBioSource product webpage