Anti-Hemoglobin subunit alpha Rabbit Monoclonal Antibody | MyBioSource MBS179210 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Hemoglobin subunit alpha Rabbit Monoclonal Antibody

catalog :

MBS179210

quantity :

0.03 mg

price :

160 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of Hemoglobin subunit alpha expression in K562 cell lysate (MBS179210). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HBA1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HBA1

product information

catalog number :

MBS179210

products type :

Antibody

products full name :

Anti-Hemoglobin subunit alpha Rabbit Monoclonal Antibody

products short name :

[Hemoglobin subunit alpha]

products name syn :

[Hemoglobin subunit alpha; Alpha-globin; Hemoglobin alpha chain; HBA1; HBA2]

other names :

[hemoglobin subunit alpha; Hemoglobin subunit alpha; hemoglobin subunit alpha; hemoglobin subunit alpha 2; Alpha-globin; Hemoglobin alpha chain]

products gene name :

[HBA1HBA2]

other gene names :

[HBA2; HBA1; HBH; HBA-T2]

clonality :

Monoclonal

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

142

purity :

Affinity-chromatography

form :

Liquid

storage stability :

Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

tested application :

Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)

app notes :

WB: 1:500-1:2000. IHC: 1:50-1:200. IP: 1:50. FC/FACS: 1:50

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human Hemoglobin subunit alpha. Tissue Specificity: Red blood cells. Conjugate: None

other info2 :

Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

products description :

Anti-Hemoglobin subunit alpha Rabbit Monoclonal Antibodytested for Flow Cytometry, IP, IHC, WB in Human, Mouse, Rat.

ncbi gi num :

4504345

ncbi acc num :

NP_000508.1

ncbi gb acc num :

NM_000517.4

uniprot acc num :

P69905

ncbi pathways :

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway (645347); Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway (645348); Malaria Pathway (152665); Malaria Pathway (152657); Metabolism Pathway (477135); O2/CO2 Exchange In Erythrocytes Pathway (645346); Scavenging Of Heme From Plasma Pathway (771600)

ncbi summary :

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. [provided by RefSeq, Jul 2008]

uniprot summary :

Involved in oxygen transport from the lung to the various peripheral tissues.

size1 :

0.03 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

340

size3 :

0.1 mg + Biotin Secondary (MBS176645)

price3 :

340

size4 :

0.1 mg + HRP Secondary (MBS176703)

price4 :

340

more info or order :

MyBioSource product webpage