Anti-Caspase-1 Rabbit Monoclonal Antibody | MyBioSource MBS179060 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Caspase-1 Rabbit Monoclonal Antibody

catalog :

MBS179060

quantity :

0.03 mg

price :

160 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

[DCB-3]

reactivity :

human, rat

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human liver, using Caspase-1 Antibody (MBS179060) CASP1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP1 Antibody (MBS179060) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (MBS176451) with DAB as the chromogen.

image 2 :

Western blot analysis of Caspase-1 in HeLa cell lysate (MBS179060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP1 monoclonal antibody (MBS179060) overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) (MBS176460) kit with Tanon 5200 system. A specific band was detected for CASP1.

product information

catalog number :

MBS179060

products type :

Antibody

products full name :

Anti-Caspase-1 Rabbit Monoclonal Antibody

products short name :

[Caspase-1]

products name syn :

[Caspase-1; CASP-1; 3.4.22.36; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45; Caspase-1 subunit p20; Caspase-1 subunit p10; CASP1; IL1BC; IL1BCE]

other names :

[caspase-1 isoform beta; Caspase-1; caspase-1; caspase 1; Interleukin-1 beta convertase; IL-1BC]

products gene name :

[CASP1]

other gene names :

[CASP1; CASP1; ICE; P45; IL1BC; IL1BC; IL1BCE; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme]

clonality :

Monoclonal

clone :

[DCB-3]

host :

Rabbit

reactivity :

Human, Rat

purity :

Affinity-chromatography

form :

Liquid; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

0.5-1mg/ml, actual concentration varies by lot. Use suggested dilution ratio to decide dilution procedure.

storage stability :

Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

tested application :

Immunohistochemistry (IHC), Western Blot (WB)

app notes :

WB: 1:500-1:2000. IHC: 1:50-1:200

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthesized peptide derived from human Caspase-1. Subcellular Localization: Cytoplasm. Tissue Specificity: Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. Conjugate: No

products categories :

Cell Biology; Apoptosis; Intracellular; Caspases Etc; Caspases

products description :

Anti-Caspase-1 Rabbit Monoclonal Antibody tested for IHC, WB in Human, Rat

ncbi gi num :

4502573

ncbi acc num :

NP_001214.1

ncbi gb acc num :

NM_001223.4

uniprot acc num :

P29466

ncbi pathways :

Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (198797); Caspase Cascade In Apoptosis Pathway (137974); Cellular Roles Of Anthrax Toxin Pathway (138076); Cytokine Signaling In Immune System Pathway (366171); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Direct P53 Effectors Pathway (137939); IFN-gamma Pathway (138040)

ncbi summary :

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]

uniprot summary :

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590).

size1 :

0.03 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

340

size3 :

0.1 mg + Biotin Secondary (MBS176645)

price3 :

340

size4 :

0.1 mg + HRP Secondary (MBS176703)

price4 :

340

more info or order :

MyBioSource product webpage