ELISA/assay

Human Fibulin-3/EFEMP1 PicoKine ELISA Kit

MBS178533

96-Strip-Wells

425 USD

ELISA Kit

Human Fibulin-3/EFEMP1 PicoKine ELISA Kit

[Fibulin-3/EFEMP1]

[DHRD; DRAD; EFEMP 1; EFEMP1; FBLN 3; FBLN3; FBNL; FIBL3; FIBL-3; FIBL 3; FIBULIN 3; FIBULIN-3; FIBULIN3; MLVT; MTLV; S1 5; T16 protein; Q12805; EGF-containing fibulin-like extracellular matrix protein 1; EGF containing fibulin-like extracellular matrix protein 1]

[EGF-containing fibulin-like extracellular matrix protein 1; EGF-containing fibulin-like extracellular matrix protein 1; EGF-containing fibulin-like extracellular matrix protein 1; EGF containing fibulin like extracellular matrix protein 1; Extracellular protein S1-5; Fibrillin-like protein; Fibulin-3; FIBL-3]

[EFEMP1]

[EFEMP1; EFEMP1; DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; FBLN3; FIBL-3; FBLN3; FBNL; FIBL-3]

FBLN3_HUMAN

Human

493

Natural and recombinant Human DPT

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).

Typical Testing Data/Standard Curve (for reference only)

Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta). Detection Range: 93.7pg/ml-6000pg/ml. Sensitivity: <10pg/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Principle of the Assay: The Picokine Human DPT Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human DPT with a 96-well strip plate that is pre-coated with antibody specific for DPT. The detection antibody is a biotinylated antibody specific for DPT. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human DPT with immunogen: Expression system for standard: NSO,Q19-V201. The kit is analytically validated with ready to use reagents. To measure Human DPT, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human DPT in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human DPT in the sample!!Background/Introduction: Dermatopontin is a protein that in humans is encoded by the DPT gene. It's an extracellular matrix protein with possible functions in cell-matrix interactions and matrix assembly. The protein is found in various tissues and many of its tyrosine residues are sulphated. Dermatopontin is postulated to modify the behavior of TGF-beta through interaction with decorin.

86788015

NP_001034437.1

NM_001039348.2

Q12805

39,195 Da

Elastic Fibre Formation Pathway (1270251); Extracellular Matrix Organization Pathway (1270244); Molecules Associated With Elastic Fibres Pathway (1270252)

This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]

EFEMP1: Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth. Defects in EFEMP1 are a cause of Doyne honeycomb retinal dystrophy (DHRD); also known as malattia leventinese (MLVT) (ML). DHRD is an autosomal dominant disease characterized by yellow-white deposits known as drusen that accumulate beneath the retinal pigment epithelium. Belongs to the fibulin family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2p16. Cellular Component: extracellular matrix; extracellular region; extracellular space; proteinaceous extracellular matrix. Molecular Function: epidermal growth factor receptor activity; epidermal growth factor receptor binding; protein binding. Biological Process: camera-type eye development; embryonic eye morphogenesis; epidermal growth factor receptor signaling pathway; negative regulation of chondrocyte differentiation; peptidyl-tyrosine phosphorylation; post-embryonic eye morphogenesis; regulation of transcription, DNA-dependent; visual perception. Disease: Doyne Honeycomb Retinal Dystrophy

96-Strip-Wells

425 USD