Anti-CIITA Antibody | MyBioSource MBS178496 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-CIITA Antibody

catalog :

MBS178496

quantity :

0.1 mg

price :

315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of CIITA expression in rat thymus extract (lane 1), mouse thymus extract (lane 2) and MCF-7 whole cell lysates (lane 3). CIITA at 123KD was detected using rabbit anti- CIITA Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.

image 2 :

CIITA was detected in paraffin-embedded sections of mouse spleen tissues using rabbit anti- CIITA Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.

image 3 :

CIITA was detected in paraffin-embedded sections of rat spleen tissues using rabbit anti- CIITA Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.

product information

catalog number :

MBS178496

products type :

Antibody

products full name :

Anti-CIITA Antibody

products short name :

[CIITA]

products name syn :

[C2TA; CIITA; CIITA IV; MHC2TA; NLRA; P33076; MHC class II transactivator; class II, major histocompatibility complex, transactivator]

other names :

[MHC class II transactivator isoform 2; MHC class II transactivator; MHC class II transactivator; class II, major histocompatibility complex, transactivator]

products gene name :

[CIITA]

other gene names :

[CIITA; CIITA; C2TA; NLRA; MHC2TA; CIITAIV; MHC2TA; CIITA]

uniprot entry name :

C2TA_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat. No cross reactivity with other proteins.

sequence length :

1130

purity :

Immunogen affinity purified.

form :

Lyophilized

storage stability :

At -20 degree C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

app notes :

Western Blot: . Concentration: 0.1-0.5 ug/ml. Tested Species: Human, Mouse, Rat. Immunohistochemisry Paraffin:. Concentration: 0.5-1ug/ml. Tested Species: Human, Mouse, Rat. Antigen Retrieval: By Heat. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

CIITA was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- CIITA Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.

other info1 :

Immunogen: E Coli-derived human CIITA recombinant protein (Position: E945-R1130). Human CIITA shares 84.4% amino acid (aa) sequence identity with mouse CIITA.

other info2 :

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

products description :

Rabbit IgG polyclonal antibody for MHC class II transactivator (CIITA) detection. Background: CIITA is a human gene which is mapped to 16p13. This gene encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the "master control factor" for the expression of these genes. Also, the protein binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction. Several transcript variants encoding different isoforms have been found for this gene.

products references :

1. Raval A, Howcroft TK, Weissman JD, Kirshner S, Zhu XS, Yokoyama K, Ting J, Singer DS (Jan 2001). "Transcriptional coactivator, CIITA, is an acetyltransferase that bypasses a promoter requirement for TAF(II)250".Molecular Cell 7 (1): 105-15. 2. Steidl C, Shah SP, Woolcock BW, Rui L, Kawahara M, Farinha P, Johnson NA, Zhao Y, Telenius A, Neriah SB, McPherson A, Meissner B, Okoye UC, Diepstra A, van den Berg A, Sun M, Leung G, Jones SJ, Connors JM, Huntsman DG, Savage KJ, Rimsza LM, Horsman DE, Staudt LM, Steidl U, Marra MA, Gascoyne RD (Mar 2011). "MHC class II transactivator CIITA is a recurrent gene fusion partner in lymphoid cancers". Nature 471 (7338): 377-81.

ncbi gi num :

156938336

ncbi acc num :

NP_000237.2

ncbi gb acc num :

NM_000246.3

uniprot acc num :

P33076

ncbi mol weight :

101,661 Da

ncbi pathways :

Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Cytokine Signaling In Immune System Pathway (1269310); Immune System Pathway (1269170); Influenza A Pathway (217173); Influenza A Pathway (217150); Interferon Signaling Pathway (1269311); Interferon Gamma Signaling Pathway (1269314); Primary Immunodeficiency Pathway (83125); Primary Immunodeficiency Pathway (537)

ncbi summary :

This gene encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the "master control factor" for the expression of these genes. The protein also binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

uniprot summary :

CIITA: a non-DNA binding transactivator that functions both in constitutive and inducible MHC Class II expression. Defects are a cause of bare lymphocyte syndrome type II (BLS II), a form of severe combined immunodeficiency disease (SCID) characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, an absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. Protein type: DNA-binding; EC 2.7.11.1; Transcription, coactivator/corepressor. Chromosomal Location of Human Ortholog: 16p13. Cellular Component: nucleoplasm; PML body. Molecular Function: DNA binding; protein binding; protein C-terminus binding; protein complex binding; transcription activator binding; transcription coactivator activity. Biological Process: immune response; negative regulation of collagen biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of MHC class I biosynthetic process; positive regulation of MHC class II biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; response to antibiotic. Disease: Bare Lymphocyte Syndrome, Type Ii; Rheumatoid Arthritis

size1 :

0.1 mg

price1 :

315 USD

more info or order :

MyBioSource product webpage