Anti-CD55 Antibody | MyBioSource MBS178344 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-CD55 Antibody

catalog :

MBS178344

quantity :

0.1 mg

price :

315 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti- CD55 Picoband antibody, MBS178344, Western blotting. All lanes: Anti CD55 (MBS178344) at 0.5ug/ml. WB: K562 Whole Cell Lysate at 40ug. Predicted bind size: 41KD. Observed bind size: 41KD

image 2 :

Anti- CD55 Picoband antibody, MBS178344, IHC(P). IHC(P): Human Placenta Tissue

product information

catalog number :

MBS178344

products type :

Antibody

products full name :

Anti-CD55 Antibody

products short name :

[CD55]

products name syn :

[Complement decay-accelerating factor; CD 55; CD55; CD55 antigen; CD55 Cromer blood group system; CD55 molecule; CD55 molecule, decay accelerating factor for complement (Cromer blood group); Cd55a; Complement decay accelerating factor; Complement decay-accelerating factor; Complement decay-accelerating factor, GPI-anchored; CR; CROM; Cromer Blood Group antigen; Cromer blood group system; DAF; Daf-GPI; DAF_HUMAN; Daf1; Dcay accelerating factor for complement (CD55, Cromer blood group system); Decay accelarating factor 1, isoform CRA_a; Decay accelerating factor (GPI-form); Decay Accelerating Factor for Complement; Decay accelerating factor GPI-form; Decay accelerating factor soluble-form; GPI-DAF; TC; CD55 molecule, decay accelerating factor for complement (Cromer blood group)]

other names :

[complement decay-accelerating factor isoform 1 preproprotein; Complement decay-accelerating factor; complement decay-accelerating factor; CD55 molecule (Cromer blood group); CD_antigen: CD55]

products gene name :

[CD55]

other gene names :

[CD55; CD55; CR; TC; DAF; CROM; CR; DAF]

uniprot entry name :

DAF_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human, Mouse

sequence length :

381

specificity :

No cross reactivity with other proteins.

purity :

Immunogen Affinity Purified

form :

Lyophilized

storage stability :

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

app notes :

Western Blot. Concentration: 0.1-0.5ug/ml. Tested Species: Hu, Ms. Immunohistochemistry (IHC) Paraffin:. Concentration: 0.5-1ug/ml. Tested Species: Hu. Antigen Retrieval: By Heat. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: E Coli-derived human CD55 recombinant protein (Position: D35-K347). Human CD55 shares 49.1% amino acid (aa) sequence identity with mouse CD55. Ig Type: Rabbit IgG

other info2 :

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

products description :

Description: Rabbit IgG polyclonal antibody for Complement decay-accelerating factor(CD55) detection. Tested with WB, IHC-P in Human;Mouse. Background: Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.

products references :

1. Medof ME, Lublin DM, Holers VM, Ayers DJ, Getty RR, Leykam JF, Atkinson JP, Tykocinski ML (April 1987). "Cloning and characterization of cDNAs encoding the complete sequence of decay-accelerating factor of human complement". Proc. Natl. Acad. Sci. U.S.A. 84 (7): 2007-11.

ncbi gi num :

10835143

ncbi acc num :

NP_000565.1

ncbi gb acc num :

NM_000574.4

uniprot acc num :

P08174

ncbi mol weight :

59,038 Da

ncbi pathways :

Asparagine N-linked Glycosylation Pathway (1268714); COPI-mediated Anterograde Transport Pathway (1339109); Class B/2 (Secretin Family Receptors) Pathway (1269570); Complement Activation, Classical Pathway (198823); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (1269241); ER To Golgi Anterograde Transport Pathway (1268726); GPCR Ligand Binding Pathway (1269544); Hematopoietic Cell Lineage Pathway (83078)

ncbi summary :

This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014]

uniprot summary :

This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.

size1 :

0.1 mg

price1 :

315 USD

more info or order :

MyBioSource product webpage