Anti-CYP24A1 Antibody | MyBioSource MBS178241 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-CYP24A1 Antibody

catalog :

MBS178241

quantity :

0.1 mg

price :

315 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti- CYP24A1 Picoband antibody, MBS178241, Western blotting. All lanes: Anti CYP24A1 (MBS178241) at 0.5ug/ml. Lane 1: Rat Thymus Tissue Lysate at 50ug. Lane 2: Rat Lung Tissue Lysate at 50ug. Lane 3: HELA Whole Cell Lysate at 40ug. Lane 4: 22RV1 Whole Cell Lysate at 40ug. Lane 5: A431 Whole Cell Lysate at 40ug. Lane 6: HEPA Whole Cell Lysate at 40ug. Predicted bind size: 59KD. Observed bind size: 59KD

image 2 :

Anti- CYP24A1 Picoband antibody, MBS178241, IHC(P). IHC(P): Rat Kidney Tissue

image 3 :

Anti- CYP24A1 Picoband antibody, MBS178241, IHC(P). IHC(P): Mouse Kidney Tissue

product information

catalog number :

MBS178241

products type :

Antibody

products full name :

Anti-CYP24A1 Antibody

products short name :

[CYP24A1]

products name syn :

[1,25-dihydroxyvitamin D(3) 24-hydroxylase; 1 25 @dihydroxyvitamin D3 24 hydroxylase; 1 25 dihydroxyvitamin D(3) 24 hydroxylase; 1; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 24 ohase; 24-OHase; 25-dihydroxyvitamin D(3) 24-hydroxylase; CP 24; CP24; CP24A_HUMAN; CYP 24; CYP24; CYP24A1; Cytochrome P450 24A1; Cytochrome P450 24A1 mitochondrial; cytochrome P450 CC24; Cytochrome P450 family 24; Cytochrome P450 family 24 subfamily A polypeptide 1; Cytochrome P450 subfamily XXIV; Cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase); Cytochrome P450-CC24; EC 1.14.13.n4; Exo mitochondrial protein; HCAI; MGC126273; MGC126274; mitochondrial; P450 CC24; Vitamin D 24 hydroxylase; Vitamin D(3) 24 hydroxylase; Vitamin D(3) 24-hydroxylase; cytochrome P450, family 24, subfamily A, polypeptide 1]

other names :

[1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial isoform 1; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; cytochrome P450 family 24 subfamily A member 1; Cytochrome P450 24A1; Cytochrome P450-CC24]

products gene name :

[CYP24A1]

other gene names :

[CYP24A1; CYP24A1; CP24; HCAI; CYP24; P450-CC24; CYP24; 24-OHase; Vitamin D(3) 24-hydroxylase]

uniprot entry name :

CP24A_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human, Mouse, Rat

sequence length :

514

specificity :

No cross reactivity with other proteins.

purity :

Immunogen Affinity Purified

form :

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

storage stability :

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

app notes :

Western Blot . Concentration: 0.1-0.5ug/ml. Tested Species : Human, Mouse, Rat . Immunohistochemistry (IHC) Paraffin. Concentration: 0.5-1ug/ml. Tested Species: Human, Mouse, Rat . Antigen Retrieval: By Heat . Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Anti- CYP24A1 Picoband antibody, MBS178241, IHC(P). IHC(P): Human Pancreatic Cancer Tissue

other info1 :

Immunogen: E Coli-derived human CYP24A1 recombinant protein (Position: E153-R514). Human CYP24A1 shares 86.2% amino acid (aa) sequence identity with both mouse and rat CYP24A1. Ig Type: Rabbit IgG

other info2 :

Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

products description :

Description: Rabbit IgG polyclonal antibody for 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial (CYP24A1) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Background: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. And this mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

products references :

1. "Entrez Gene: CYP24A1 cytochrome P450, family 24, subfamily A, polypeptide 1". 2. Dauber A, Nguyen TT, Sochett E, Cole DE, Horst R, Abrams SA, Carpenter TO, Hirschhorn JN (February 2012). "Genetic defect in CYP24A1, the vitamin D 24-hydroxylase gene, in a patient with severe infantile hypercalcemia". J. Clin. Endocrinol. Metab. 97 (2): E268-74. 3. Sakaki T, Kagawa N, Yamamoto K, Inouye K (January 2005). "Metabolism of vitamin D3 by cytochromes P450". Front. Biosci. 10: 119-34.

ncbi gi num :

55770850

ncbi acc num :

NP_000773.2

ncbi gb acc num :

NM_000782.4

uniprot acc num :

Q07973

ncbi mol weight :

43,051 Da

ncbi pathways :

Biological Oxidations Pathway (1270189); Cytochrome P450 - Arranged By Substrate Type Pathway (1270191); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Fat-soluble Vitamins Pathway (1339147); Metabolism Of Vitamins And Cofactors Pathway (1270144); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); Nuclear Receptors In Lipid Metabolism And Toxicity Pathway (198887); Phase 1 - Functionalization Of Compounds Pathway (1270190)

ncbi summary :

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

CYP24A1: Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23- hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product. Defects in CYP24A1 are the cause of hypercalcemia infantile (HCAI). HCAI is a disorder characterized by abnormally high level of calcium in the blood, failure to thrive, vomiting, dehydration, and nephrocalcinosis. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Oxidoreductase; EC 1.14.13.126; Mitochondrial. Chromosomal Location of Human Ortholog: 20q13. Cellular Component: mitochondrial inner membrane; mitochondrion; nucleoplasm; nucleus; plasma membrane. Molecular Function: 1-alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3) 24-hydroxylase activity; 25-hydroxycholecalciferol-24-hydroxylase activity; heme binding; iron ion binding; oxidoreductase activity. Biological Process: osteoblast differentiation; response to vitamin D; vitamin D catabolic process; vitamin D metabolic process; vitamin metabolic process. Disease: Hypercalcemia, Infantile

size1 :

0.1 mg

price1 :

315 USD

more info or order :

MyBioSource product webpage