Anti-SAP97 Antibody | MyBioSource MBS178053 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-SAP97 Antibody

catalog :

MBS178053

quantity :

0.1 mg

price :

315 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti- SAP97 Picoband antibody, MBS178053, Western blotting. All lanes: Anti SAP97 (MBS178053) at 0.5ug/ml. Lane 1: Rat Lung Tissue Lysate at 50ug. Lane 2: Mouse Lung Tissue Lysate at 50ug. Lane 3: HELA Whole Cell Lysate at 40ug. Lane 4: MM231 Whole Cell Lysate at 40ug. Lane 5: COLO320 Whole Cell Lysate at 40ug. Lane 6: A549 Whole Cell Lysate at 40ug. Lane 7: NIH3T3 Whole Cell Lysate at 40ug. Predicted bind size: 130KD. Observed bind size: 130KD

image 2 :

Anti- SAP97 Picoband antibody, MBS178053, IHC(P). IHC(P): Human Prostatic Cancer Tissue

product information

catalog number :

MBS178053

products type :

Antibody

products full name :

Anti-SAP97 Antibody

products short name :

[SAP97]

products name syn :

[Disks large homolog 1; Discs large homolog 1; discs large, Drosophila, homolog of, 1; discs, large homolog 1 (Drosophila); Disks large homolog 1; dJ1061C18.1.1; DKFZp761P0818; DKFZp781B0426; DLG 1; DLG1; DLG1_HUMAN; DLGH 1; DLGH1; HDLG; Presynaptic protein SAP97; SAP 97; SAP-97; SAP97; Synapse associated protein 97; Synapse-associated protein 97; discs, large homolog 1 (Drosophila)]

other names :

[disks large homolog 1 isoform 1; Disks large homolog 1; disks large homolog 1; discs large homolog 1, scribble cell polarity complex component; Synapse-associated protein 97; SAP-97; SAP97; hDlg]

products gene name :

[SAP97]

other gene names :

[DLG1; DLG1; hdlg; DLGH1; SAP97; SAP-97; dJ1061C18.1.1; SAP-97; SAP97]

uniprot entry name :

DLG1_HUMAN

clonality :

Polyclonal

host :

unknown

reactivity :

Human, Mouse, Rat

sequence length :

904

specificity :

No cross reactivity with other proteins.

purity :

Immunogen Affinity Purified

form :

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

storage stability :

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

app notes :

Western Blot. Concentration: 0.1-0.5ug/ml. Tested Species: Hu, MS, Rat. Immunohistochemistry (Paraffin-embedded Section). Concentration: 0.5-1ug/ml. Tested Species: Hu. Antigen Retrieval: By Heat. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: E Coli-derived human SAP97 recombinant protein (Position: M1-A165). Human SAP97 shares 89.7% and 84.2% amino acid (aa) sequence identity with mouse and rat SAP97, respectively. Ig Type: Rabbit IgG

other info2 :

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

products description :

Description: Rabbit IgG polyclonal antibody for Disks large homolog 1(DLG1) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Background: Disks large homolog 1 (DLG1), also known as synapse-associated protein 97 or SAP97, is a protein that in humans is encoded by the SAP97 gene. SAP97 is expressed throughout the body in epithelial cells, including the kidney and brain. There is some evidence that SAP97 regulates cell-to-cell adhesion during cell death, and may interact with HPV. In the brain, SAP97's function is involved in the trafficking of transmembrane receptors from the ER to the plasma membrane. SAP97's function has been investigated by reducing its expression by knockout or increasing its expression heterologously. Mice in which the SAP97 gene has been knocked out die perinatally, have a cleft palate, and deficiencies in renal function. Overexpression of SAP97 in mammalian neurons leads to increased synaptic strength.

products references :

1. Müller BM; Kistner U; Veh RW et al. (March 1995). "Molecular characterization and spatial distribution of SAP97, a novel presynaptic protein homologous to SAP90 and the Drosophila discs-large tumor suppressor protein". J. Neurosci. 15 (3 Pt 2): 2354-66. 2. umbaugh G, Sia GM, Garner CC, Huganir RL (June 2003). "Synapse-associated protein-97 isoform-specific regulation of surface AMPA receptors and synaptic function in cultured neurons". J. Neurosci. 23 (11): 4567-76. 3. Sans N, Racca C, Petralia RS, Wang YX, McCallum J, Wenthold RJ (October 2001). "Synapse-associated protein 97 selectively associates with a subset of AMPA receptors early in their biosynthetic pathway". J. Neurosci. 21 (19): 7506-16.

ncbi gi num :

148539628

ncbi acc num :

NP_001091894.1

ncbi gb acc num :

NM_001098424.1

uniprot acc num :

Q12959

ncbi mol weight :

88,485 Da

ncbi pathways :

Activation Of Ca-permeable Kainate Receptor Pathway (1268811); Activation Of Kainate Receptors Upon Glutamate Binding Pathway (1268808); Axon Guidance Pathway (1270303); BDNF Signaling Pathway (712093); CDC42 Signaling Events Pathway (137994); Developmental Biology Pathway (1270302); E-cadherin Signaling In The Nascent Adherens Junction Pathway (138005); Glutamate Binding, Activation Of AMPA Receptors And Synaptic Plasticity Pathway (1268794); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889)

ncbi summary :

This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known. [provided by RefSeq, Feb 2011]

uniprot summary :

Essential multidomain scaffolding protein required for normal development (). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel.

size1 :

0.1 mg

price1 :

315 USD

more info or order :

MyBioSource product webpage