Anti-Aquaporin 4 Antibody | MyBioSource MBS178038 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Aquaporin 4 Antibody

catalog :

MBS178038

quantity :

0.1 mg

price :

315 USD

clonality :

polyclonal

host :

unidentified

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti- Aquaporin 4 Picoband antibody, MBS178038 Western blotting. All lanes: Anti Aquaporin 4 (MBS178038) at 0.5ug/ml. Lane 1: Rat Brain Tissue Lysate at 50ug. Lane 2: Mouse Brain Tissue Lysate at 50ug. Predicted bind size: 35KD. Observed bind size: 45KD

image 2 :

Anti-Aquaporin 4 Picoband antibody, MBS178038, IHC(P). IHC(P): Mouse Brain Tissue

image 3 :

Anti-Aquaporin 4 Picoband antibody, MBS178038, IHC(P). IHC(P): Rat Brain Tissue

product information

catalog number :

MBS178038

products type :

Antibody

products full name :

Anti-Aquaporin 4 Antibody

products short name :

[Aquaporin 4]

products name syn :

[Aquaporin-4; AQP 4; AQP-4; AQP4; AQP4_HUMAN; Aquaporin type 4; Aquaporin-4; Aquaporin4; HMIWC 2; HMIWC2; Mercurial insensitive water channel; Mercurial-insensitive water channel; MGC22454; MIWC; WCH 4; WCH4; aquaporin 4]

other names :

[aquaporin-4 isoform M1; Aquaporin-4; aquaporin-4; aquaporin 4; Mercurial-insensitive water channel; MIWC; WCH4]

other gene names :

[AQP4; AQP4; MIWC; WCH4; AQP-4; MIWC]

uniprot entry name :

AQP4_HUMAN

clonality :

Polyclonal

isotype :

Rabbit IgG

host :

unknown

reactivity :

Human, Mouse, Rat

sequence length :

323

specificity :

No cross reactivity with other proteins

purity :

Immunogen Affinity Purified

form :

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg NaN 3

storage stability :

Store at -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin

app notes :

Western Blot . Concentration: 0.1-0.5ug/ml. Tested Species: Ms, Rat. Predicted Species: Hu. Antigen Retrieval: N/A. Immunohistochemistry (Paraffin-embedded Section): . Concentration: 0.5-1ug/ml. Tested Species: Hu, Ms, Rat. Predicted Species: N/A. Antigen Retrieval: By Heat. Immunohistochemistry (Frozen Section): . Concentration: 0.5-1ug/ml. Tested Species: Ms, Rat. Predicted Species: Hu. Antigen Retrieval: N/A. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by ens users.

image1 heading :

Western Blot

image2 heading :

Immunohistochemistry

image3 heading :

Immunohistochemistry

image4 heading :

Immunohistochemistry

image4 description :

Anti-Aquaporin 4 Picoband antibody, MBS178038, IHC(P). IHC(P): Human Glioma Tissue

image5 heading :

Immunohistochemistry

image5 description :

Anti-Aquaporin 4 Picoband antibody, MBS178038, IHC(F). IHC(F): Mouse Brain Tissue

other info1 :

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4 (295-323aa KPGVVHVIDVDRGEEKKGKDQSGEVLSSV), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

other info2 :

Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

products description :

Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provides a pathway for water to exit these cells. The gene of AQP4 is mapped to 18q11.2-q12.1. Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127, 55, 27, and 92 amino acids separated by introns of 0.8, 0.3, and 5.2 kb. Unlike other aquaporins, an alternative coding initiation sequence (designated exon 0) was located 2.7 kb upstream of exon 1. When spliced together, M1 and the subsequent 10 amino acids are encoded by exon 0; the next 11 amino acids and M23 are encoded by exon 1. AQP4 is expressed in astrocytes and is upregulated by direct insult to the central nervous system. And AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier.

products references :

1. Amiry-Moghaddam, M.; Otsuka, T.; Hurn, P. D.; Traystman, R. J.; Haug, F.-M.; Froehner, S. C.; Adams, M. E.; Neely, J. D.; Agre, P.; Ottersen, O. P.; Bhardwaj, A. : An alpha-syntrophin-dependent pool of AQP4 in astroglial end-feet confers bidirectional water flow between blood and brain. Proc. Nat. Acad. Sci. 100: 2106-2111, 2003. 2. Agre P, Nielsen S (1996). "The aquaporin family of water channels in kidney". Nephrologie 17 (7): 409-15. 3. Lennon, V. A.; Kryzer, T. J.; Pittock, S. J.; Verkman, A. S.; Hinson, S. R. : IgG marker of optic-spinal multiple sclerosis binds to the aquaporin-4 water channel. J. Exp. Med. 202: 473-477, 2005. 4. Lu, M.; Lee, M. D.; Smith, B. L.; Jung, J. S.; Agre, P.; Verdijk, M. A. J.; Merkx, G.; Rijss, J. P. L.; Deen, P. M. T. : The human AQP4 gene: definition of the locus encoding two water channel polypeptides in brain. Proc. Nat. Acad. Sci. 93: 10908-10912, 1996. 5. Nagelhus EA, Mathiisen TM, Ottersen OP (2004). "Aquaporin-4 in the central nervous system: cellular and subcellular distribution and coexpression with KIR4.1". Neuroscience 129 (4): 905-13.

ncbi gi num :

4502181

ncbi acc num :

NP_001641.1

ncbi gb acc num :

NM_001650.5

uniprot acc num :

P55087

ncbi pathways :

Aquaporin-mediated Transport Pathway (1269944); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Passive Transport By Aquaporins Pathway (1269945); SIDS Susceptibility Pathways (198901); Spinal Cord Injury Pathway (739007); Transmembrane Transport Of Small Molecules Pathway (1269903); Vasopressin Regulates Renal Water Homeostasis Via Aquaporins Pathway (1269946); Vasopressin-regulated Water Reabsorption Pathway (143700); Vasopressin-regulated Water Reabsorption Pathway (143631)

ncbi summary :

This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2015]

uniprot summary :

AQP4: a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The predominant aquaporin found in brain. Two alternatively spliced transcript variants have been described. Protein type: Transporter, aquaporin family; Transporter; Channel, misc.; Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 18q11.2-q12.1. Cellular Component: basolateral plasma membrane; cytoplasm; external side of plasma membrane; integral to plasma membrane; plasma membrane. Molecular Function: glycerol channel activity; water channel activity; water transporter activity. Biological Process: cellular water homeostasis; glycerol transport; multicellular organismal water homeostasis; renal water homeostasis; transport; water transport

size1 :

0.1 mg

price1 :

315 USD

more info or order :

MyBioSource product webpage