Anti-ABCB4 Antibody | MyBioSource MBS177897 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-ABCB4 Antibody

catalog :

MBS177897

quantity :

0.1 mg

price :

315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Figure 1. Western blot analysis of ABCB4 using anti-ABCB4 antibody (MBS177897). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate,. Lane 2: SW620 Whole Cell Lysate,. Lane 3: 22RV1 Whole Cell Lysate,. Lane 4: SKOV Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB4 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ABCB4 at approximately 142KD. The expected band size for ABCB4 is at 142KD.

image 2 :

Figure 2. IHC analysis of ABCB4 using anti-ABCB4 antibody (MBS177897). ABCB4 was detected in paraffin-embedded section of Mouse Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ABCB4 Antibody (MBS177897) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

image 3 :

Figure 3. IHC analysis of ABCB4 using anti-ABCB4 antibody (MBS177897). ABCB4 was detected in paraffin-embedded section of Rat Liver Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ABCB4 Antibody (MBS177897) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

product information

catalog number :

MBS177897

products type :

Antibody

products full name :

Anti-ABCB4 Antibody

products short name :

[ABCB4]

products name syn :

[Phosphatidylcholine translocator ABCB4;Multidrug resistance protein 3; ABC 21; ABC B4; ABC21; ABCB 4; Abcb4; ABCB4 protein; ATP binding cassette sub family B MDR/TAP member 4; ATP binding cassette sub family B member 4; ATP-binding cassette sub-family B member 4; MDR 3; MDR2; MDR2/3; MDR3; MDR3 P glycoprotein; MDR3 P gp; MDR3_HUMAN; Multidrug resistance protein 3; Multiple drug resistance 3; P glycoprotein 3; P-glycoprotein 3; PFIC 3; PFIC3; PGY 3; PGY3; ATP-binding cassette, sub-family B (MDR/TAP), member 4]

other names :

[phosphatidylcholine translocator ABCB4 isoform A; Phosphatidylcholine translocator ABCB4; phosphatidylcholine translocator ABCB4; ATP binding cassette subfamily B member 4; ATP-binding cassette sub-family B member 4]

products gene name :

[ABCB4]

other gene names :

[ABCB4; ABCB4; GBD1; ICP3; MDR2; MDR3; PGY3; ABC21; MDR2/3; PFIC-3]

uniprot entry name :

MDR3_HUMAN

clonality :

Polyclonal

isotype :

Rabbit IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

1279

specificity :

No cross reactivity with other proteins.

purity :

Immunogen Affinity Purified

form :

Lyophilized

storage stability :

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

app notes :

Western Blot:. Concentration: 0.1-0.5ug/ml. Tested Species: Hu. Immunohistochemistry (Paraffin-embedded Section): . Concentration: 0.5-1ug/ml. Tested Species: Hu, Ms, Rat. Antigen Retrieval: By Heat. Immunohistochemistry (Frozen section):. Concentration: 0.5-1ug/ml. Tested Species: Hu. Immunocytochemistry:. Concentration: 0.5-1ug/ml. Tested Species: Hu. Flow Cytometry:. 1-3ug/1x10^6 cels. Tested Species: Hu. WB: The detection limit for ABCB4 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Figure 4. IHC analysis of ABCB4 using anti-ABCB4 antibody (MBS177897). ABCB4 was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ABCB4 Antibody (MBS177897) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

image5 heading :

Flow Cytometry (FC/FACS)

image5 description :

Figure 5. Flow Cytometry analysis of A375 cells using anti-MDR3 antibody (MBS177897). Overlay histogram showing A375 cells stained with MBS177897 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MDR3 Antibody (MBS177897,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

other info1 :

Immunogen: E Coli-derived human ABCB4 recombinant protein (Position: A601-A720). Human ABCB4 shares 79% amino acid (aa) sequence identity with both mouse and rat ABCB4.

other info2 :

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg NaN 3 .

products description :

Description: Rabbit IgG polyclonal antibody for Multidrug resistance protein 3(ABCB4) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Background: Adenosine triphosphate-binding cassette subfamily B, member 4 (ABCB4), also called MDR3, is a protein that in humans is encoded by the ABCB4 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. The ABCB4 gene contains 28 exons and 27 of these contain coding sequences for the two homologous halves of the protein that correlate with functional domains. ABCB4 gene mutations represent a genetic factor involved in this peculiar form of cholesterol gallstone disease in adults.

products references :

1. Lincke, C. R., Smit, J. J., van der Velde Koerts, T., Borst, P. Structure of the human MDR3 gene and physical mapping of the human MDR locus. J. Biol. Chem. 266: 5303-5310, 1991. 2. Rosmorduc, O., Hermelin, B., Poupon, R. MDR3 gene defect in adults with symptomatic intrahepatic and gallbladder cholesterol cholelithiasis. Gastroenterology 120: 1459-1467, 2001.

ncbi gi num :

4505771

ncbi acc num :

NP_000434.1

ncbi gb acc num :

NM_000443.3

uniprot acc num :

P21439

ncbi pathways :

ABC Transporters Pathway (83035); ABC Transporters Pathway (436); ABC-family Proteins Mediated Transport Pathway (1269904); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (1270010); Metabolism Pathway (1269956); Metabolism Of Lipids And Lipoproteins Pathway (1270001); Nuclear Receptors In Lipid Metabolism And Toxicity Pathway (198887); PPARA Activates Gene Expression Pathway (1270030)

ncbi summary :

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function. [provided by RefSeq, Jul 2008]

uniprot summary :

ABCB4: a transporter that mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Human ABCB4 is not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte. Interacts with HAX1. Defects are the cause of gallstones, and progressive familial intrahepatic cholestasis 3, an autosomal recessive liver disorder that progresses to cirrhosis and liver failure before adulthood. Two alternatively spliced human isoforms have been described. Protein type: Transporter, ABC family; EC 3.6.3.44; Hydrolase; Transporter; Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 7q21.1. Cellular Component: apical plasma membrane; clathrin-coated vesicle; cytoplasm; integral to plasma membrane; intercellular canaliculus; lipid raft; membrane; plasma membrane. Molecular Function: ATP binding; ATPase activity, coupled to transmembrane movement of substances; phosphatidylcholine transmembrane transporter activity; phospholipid transporter activity; protein binding; xenobiotic-transporting ATPase activity. Biological Process: antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent; antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent; bile acid secretion; lipid homeostasis; lipid metabolic process; multidrug transport; phospholipid translocation; positive regulation of antigen processing and presentation of peptide antigen via MHC class I; positive regulation of cholesterol transport; response to drug; transmembrane transport; transport; xenobiotic transport. Disease: Cholestasis, Intrahepatic, Of Pregnancy 3; Cholestasis, Progressive Familial Intrahepatic, 3; Gallbladder Disease 1

size1 :

0.1 mg

price1 :

315 USD

more info or order :

MyBioSource product webpage