antibody

Anti-FOXP3 Antibody

MBS177894

0.1 mg

315 USD

polyclonal

unidentified

nonconjugated

human, mouse, rat

immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Anti-FOXP3 Antibody

[FOXP3]

[Forkhead box protein P3; AIID; DIETER; Forkhead box P3; Forkhead box protein P3; foxp3; FOXP3_HUMAN; FOXP3delta7; Immune dysregulation polyendocrinopathy enteropathy X linked; Immunodeficiency polyendocrinopathy enteropathy X linked; IPEX; JM2; MGC141961; MGC141963; OTTHUMP00000025832; OTTHUMP00000025833; OTTHUMP00000226737; PIDX; SCURFIN; XPID; forkhead box P3]

[forkhead box protein P3 isoform b; Forkhead box protein P3; forkhead box protein P3; forkhead box P3; Scurfin]

[FOXP3]

[FOXP3; FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER; IPEX]

FOXP3_HUMAN

Polyclonal

Rabbit IgG

unknown

Human, Mouse, Rat

396

No cross reactivity with other proteins

Immunogen Affinity Purified

Lyophilized

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Immunohistochemistry (IHC) Paraffin

Concentration: 0.5-1ug/ml. Tested Species: Hu, Ms, Rat. Antigen Retrieval: By Heat. Tested Species: In house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications not tested. Optimal dilutions should be determined by end users.

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunogen: E Coli-derived human FOXP3 recombinant protein (Position: H101-P431). Human FOXP3 shares 88% amino acid (aa) sequence identity with mouse FOXP3. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg NaN 3 .

Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Background: FOXP3(forkhead box P3) is a protein involved in immune system responses. The human FOXP3 genes contain 11 coding exons. Exon-intron boundaries are identical across the coding regions of the mouse and human genes. By genomic sequence analysis, the FOXP3 gene maps to the p arm of the X chromosome(specifically, Xp11.23). A member of the FOX protein family, FOXP3 appears to function as a master regulator in the development and function of regulatory T cells. While the precise control mechanism has not yet been established, FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription.

1. Allan, S. E., Passerini, L., Bacchetta, R., Crellin, N., Dai, M., Orban, P. C., Ziegler, S. F., Roncarolo, M. G., Levings, M. K.The role of 2 FOXP3 isoforms in the generation of human CD4+ Tregs.J. Clin. Invest. 115: 3276-3284, 2005. 2. Bennett, C. L., Christie, J., Ramsdell, F., Brunkow, M. E., Ferguson, P. J., Whitesell, L., Kelly, T. E., Saulsbury, F. T., Chance, P. F., Ochs, H. D.The immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) is caused by mutations of FOXP3.Nature Genet. 27: 20-21, 2001. 3. Hong, J., Li, N., Zhang, X., Zheng, B., Zhang, J. Z.Induction of CD4+CD25+ regulatory T cells by copolymer-I through activation of transcription factor Foxp3.Proc. Nat. Acad. Sci. 102: 6449-6454, 2005.

167466190

NP_001107849.1

NM_001114377.1

Q9BZS1

Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); IL2 Signaling Events Mediated By STAT5 Pathway (137988); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797)

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

FOXP3: Probable transcription factor. Plays a critical role in the control of immune response. Interacts with IKZF3. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cell cycle regulation; DNA-binding; C2H2-type zinc finger protein; Transcription factor. Chromosomal Location of Human Ortholog: Xp11.23. Cellular Component: cytoplasm; nucleus; protein complex. Molecular Function: histone acetyltransferase binding; histone deacetylase binding; metal ion binding; NF-kappaB binding; NFAT protein binding; protein binding; protein homodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity. Biological Process: B cell homeostasis; CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment; chromatin remodeling; cytokine production; inhibition of CREB transcription factor; inhibition of NF-kappaB transcription factor; myeloid cell homeostasis; negative regulation of activated T cell proliferation; negative regulation of cell proliferation; negative regulation of chronic inflammatory response; negative regulation of cytokine biosynthetic process; negative regulation of cytokine secretion; negative regulation of histone acetylation; negative regulation of histone deacetylation; negative regulation of immune response; negative regulation of interferon-gamma biosynthetic process; negative regulation of interferon-gamma production; negative regulation of interleukin-10 production; negative regulation of interleukin-17 production; negative regulation of interleukin-2 biosynthetic process; negative regulation of interleukin-2 production; negative regulation of interleukin-4 production; negative regulation of interleukin-5 production; negative regulation of interleukin-6 production; negative regulation of isotype switching to IgE isotypes; negative regulation of T cell cytokine production; negative regulation of T cell proliferation; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of tumor necrosis factor production; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; positive regulation of histone acetylation; positive regulation of immature T cell proliferation in the thymus; positive regulation of interleukin-4 production; positive regulation of peripheral T cell tolerance induction; positive regulation of T cell anergy; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor-beta1 production; regulation of isotype switching to IgG isotypes; regulation of T cell anergy; regulation of transcription, DNA-dependent; response to virus; T cell activation; T cell homeostasis; T cell mediated immunity; T cell receptor signaling pathway; tolerance induction to self antigen; transcription, DNA-dependent. Disease: Diabetes Mellitus, Insulin-dependent; Immunodysregulation, Polyendocrinopathy, And Enteropathy, X-linked

0.1 mg

315 USD