Anti-TGM2 Antibody | MyBioSource MBS177799 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-TGM2 Antibody

catalog :

MBS177799

quantity :

0.1 mg

price :

315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti- TGM2 Picoband antibody, MBS177799, Western blotting. All lanes: Anti TGM2 (MBS177799) at 0.5ug/ml. Lane 1: Rat Liver Tissue Lysate at 50ug. Lane 2: Rat Ovary Tissue Lysate at 50ug. Lane 3: HELA Whole Cell Lysate at 40ug. Predicted bind size: 77KD. Observed bind size: 77KD

image 2 :

Anti- TGM2 Picoband antibody, MBS177799, IHC(P). IHC(P): Mouse Spleen Tissue

image 3 :

Anti- TGM2 Picoband antibody, MBS177799, IHC(P). IHC(P): Rat Spleen Tissue

product information

catalog number :

MBS177799

products type :

Antibody

products full name :

Anti-TGM2 Antibody

products short name :

[TGM2]

products name syn :

[Protein-glutamine gamma-glutamyltransferase 2; ALPHA SUBUNIT; C polypeptide; EC 2.3.2.13; G alpha h; G protein alpha subunit Gh class; G[a]h; Gh CLASS G ALPHA h; GNAH; GNAH G PROTEIN; Guanine nucleotide binding protein H polypeptide; H POLYPEPTIDE; Protein glutamine gamma glutamyltransferase 2; Protein glutamine gamma glutamyltransferase; Protein-glutamine gamma-glutamyltransferase 2; TG 2; TG(C); TG2; TGase C; TGase H; TGase-2; TgaseII; TGC; TGC GUANINE NUCLEOTIDE BINDING PROTEIN; TGM 2; TGM2; TGM2_HUMAN; Tissue transglutaminase; Tissue type transglutaminase; Transglutaminase 2; Transglutaminase 2 C polypeptide; Transglutaminase C; Transglutaminase H; Transglutaminase-2; tTG; tTGas antibody; transglutaminase 2]

other names :

[protein-glutamine gamma-glutamyltransferase 2 isoform a; Protein-glutamine gamma-glutamyltransferase 2; protein-glutamine gamma-glutamyltransferase 2; transglutaminase 2; Tissue transglutaminase; Transglutaminase C; TG(C); TGC; TGase C; Transglutaminase H; TGase H; Transglutaminase-2; TGase-2]

products gene name :

[TGM2]

other gene names :

[TGM2; TGM2; TGC; TG(C); TG(C); TGC; TGase C; TGase H; TGase-2]

uniprot entry name :

TGM2_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

687

specificity :

No cross reactivity with other proteins.

purity :

Immunogen Affinity Purified

form :

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

storage stability :

At -20° for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin

app notes :

Western Blot . Concentration: 0.1-0.5 ug/ml. Tested Species: Human, Rat. Immunohistochemistry (Paraffin-embedded Section) . Concentration: 0.5-1 ug/ml. Tested Species: Human, Mouse, Rat. Antigen Retrieval: By Heat. Immunohistochemistry (Frozen Section): . Concentration: 0.5-1 ug/ml. Tested Species: Human, Mouse, Rat. . WB: The detection limit for TMG2 is approximately 0.1 ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0 for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Anti- TGM2 Picoband antibody, MBS177799, IHC(P). IHC(P): Human Intestinal Cancer Tissue

image5 heading :

Immunohistochemistry (IHC)

image5 description :

Anti- TGM2 Picoband antibody, MBS177799, IHC(P). IHC(P): Human Mammary Cancer Tissue

other info1 :

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human TGM2 (7-34aa LERCDLELETNGRDHHTADLCREKLVVR), different from the related mouse sequence by five amino acids. Ig Type: Rabbit IgG

other info2 :

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

products description :

Description: Rabbit IgG polyclonal antibody for Protein-glutamine gamma-glutamyltransferase 2(TGM2) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat. Background: Tissue transglutaminase, also called TGC, is a 78-kDa, calcium dependent enzyme of the protein-glutamine gamma-glutamyltransferases family. By fluorescence in situ hybridization (FISH), this gene is mapped in 20q11.23. Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease.

products references :

1. Antonyak, M. A., Jansen, J. M., Miller, A. M., Ly, T. K., Endo, M., Cerione, R. A. Two isoforms of tissue transglutaminase mediate opposing cellular fates. Proc. Nat. Acad. Sci. 103: 18609-18614, 2006. 2. Mastroberardino, P. G., Iannicola, C., Nardacci, R., Bernassola, F., de Laurenzi, V., Melino, G., Moreno, S., Pavone, F., Oliverio, S., Fesus, L., Piacentini, M. 'Tissue' transglutaminase ablation reduces neuronal death and prolongs survival in a mouse model of Huntington's disease. Cell Death Differ. 9: 873-880, 2002. 3. Priglinger, S. G., May, C. A., Neubauer, A. S., Alge, C. S., Schoenfeld, C.-L., Kampik, A., Welge-Lussen, U. Tissue transglutaminase as a modifying enzyme of the extracellular matrix in PVR membranes. Invest. Ophthal. Vis. Sci. 44: 355-364, 2003.

ncbi gi num :

39777597

ncbi acc num :

NP_004604.2

ncbi gb acc num :

NM_004613.3

uniprot acc num :

P21980

ncbi pathways :

Huntington's Disease Pathway (83100); Huntington's Disease Pathway (512); Thromboxane A2 Receptor Signaling Pathway (138060)

ncbi summary :

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

TGM2: an enzyme of the transglutaminase family that catalyzes the crosslinking of proteins and the conjugation of polyamines to proteins. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Is the autoantigen in coeliac disease and plays a role in apoptosis, cellular differentiation and matrix stabilisation. Three alternatively spliced isoforms have been described. Protein type: EC 2.3.2.13; Transferase. Chromosomal Location of Human Ortholog: 20q12. Cellular Component: cytosol; endoplasmic reticulum; focal adhesion; intrinsic to plasma membrane; mitochondrion. Molecular Function: metal ion binding; protein binding; protein-glutamine gamma-glutamyltransferase activity. Biological Process: apoptotic cell clearance; elevation of mitochondrial calcium ion concentration; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); peptide cross-linking; positive regulation of apoptosis; positive regulation of cell adhesion; reduction of endoplasmic reticulum calcium ion concentration

size1 :

0.1 mg

price1 :

315 USD

more info or order :

MyBioSource product webpage