antibody

Anti-PROM1 Antibody

MBS177458

0.1 mg

280 USD

polyclonal

rabbit

nonconjugated

western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Anti-PROM1 Antibody

Prominin-1

AC133; Antigen AC133; CD133 antibody; CORD12; hematopoietic stem cell antigen; hProminin; MCDR2; MSTP061 antibody; PROM1; PROM 1; PROM-1; Prominin-1; Prominin 1; Prominin1; Prominin-like protein 1; PROML1 antibody; RP41; STGD4; prominin 1

prominin-1 isoform 2; Prominin-1; prominin-1; hProminin; antigen AC133; prominin-like protein 1; hematopoietic stem cell antigen; prominin 1; Antigen AC133; Prominin-like protein 1; CD_antigen: CD133

PROM1

PROM1; PROM1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061; PROML1

PROM1_HUMAN

Polyclonal

IgG

Rabbit

Human

856

No cross reactivity with other proteins.

Immunogen affinity purified.

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB), Immunohistochemistry (IHC-P)

Western Blot: . Concentration: 0.1-0.5mug/ml; . Tested Species: Human. Antigen Retrival: - . Immunohistochemistry (Paraffin-embedded Section): . Concentration: 0.5-1mug/ml; Tested Species: Human . Antigen Retrieval: By Heat. WB: The detection limit for PROM1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the Paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Immunogen: E.coli-derived human PROM1 recombinant protein (Position: P531-H865). Human PROM1 shares 61% amino acid (aa) sequence identity with mouse PROM1.

Description: Rabbit IgG polyclonal antibody for Prominin-1(PROM1) detection. Tested with WB, IHC-P in Human. Background: Prominin-1, also known as CD133, is a glycoprotein that in humans is encoded by the PROM1 gene. It is mapped to 4p15.32. Prominin-1 is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. It has been proposed to act as an organizer of cell membrane topology. Prominin-1 was expressed not only on metastatic colon cancer cells, but also on differentiated colonic epithelium in both adult mice and humans.

224994189

NP_001139319.1

NM_001145847.1

O43490

94,605 Da

Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

CD133: Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. Interacts with CDHR1 and with actin filaments. Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells. Expressed in adult retina by rod and cone photoreceptor cells. Belongs to the prominin family. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Cell surface; Membrane protein, integral. Chromosomal Location of Human Ortholog: 4p15.32. Cellular Component: extracellular space; stereocilium; cell surface; photoreceptor outer segment; integral to plasma membrane; endoplasmic reticulum; microvillus membrane; apical plasma membrane; plasma membrane; ER-Golgi intermediate compartment; brush border; vesicle. Molecular Function: actinin binding; protein binding; cadherin binding. Biological Process: photoreceptor cell maintenance; retina morphogenesis in camera-type eye. Disease: Macular Dystrophy, Retinal, 2; Stargardt Disease 4; Retinitis Pigmentosa 41; Cone-rod Dystrophy 12

0.1 mg

280 USD