Human NOV/CCN3 PicoKine ELISA Kit | MyBioSource MBS177346 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human NOV/CCN3 PicoKine ELISA Kit

catalog :

MBS177346

quantity :

96-Strip-Wells

price :

425 USD

more info or order :

MyBioSource product webpage

image

image 1 :

Human NOV/CCN3 PicoKine ELISA Kit standard curve

product information

catalog number :

MBS177346

products type :

ELISA Kit

products full name :

Human NOV/CCN3 PicoKine ELISA Kit

products short name :

[NOV/CCN3]

products name syn :

[Protein NOV homolog; nephroblastoma overexpressed; CCN family member 3; CCN3; IBP-9; IGF-binding protein 9; IGFBP-9; IGFBP9; Insulin-like growth factor-binding protein 9; Nephroblastoma overexpressed; Nephroblastoma overexpressed gene protein homolog; Nephroblastoma-overexpressed gene protein homolog; NOV; NOV_HUMAN; NovH; Protein NOV homolog]

other names :

[Protein NOV homolog; Protein NOV homolog; protein NOV homolog; CCN family member 3; IGF-binding protein 9; insulin-like growth factor-binding protein 9; nephroblastoma-overexpressed gene protein homolog; nephroblastoma overexpressed; CCN family member 3; Insulin-like growth factor-binding protein 9; IBP-9; IGF-binding protein 9; IGFBP-9; Nephroblastoma-overexpressed gene protein homolog]

products gene name :

[NOV]

other gene names :

[NOV; NOV; CCN3; NOVh; IBP-9; IGFBP9; IGFBP-9; CCN3; IGFBP9; NOVH; NovH; IBP-9; IGF-binding protein 9; IGFBP-9]

uniprot entry name :

NOV_HUMAN

reactivity :

Human

sequence length :

357

specificity :

Natural and recombinant Human NOV/CCN3

storage stability :

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Assay Type: Quantitative Sandwich. Detection Range: 31.2pg/ml-2000pg/ml. Sensitivity: <2pg/ml

other info2 :

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

products description :

Principle of the Assay: Human NOV/CCN3 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for NOV/CCN3 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: T32-M357) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NOV/CCN3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human NOV/CCN3 amount of sample captured in plate. Background/Introduction: NOV(nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the diagnosis of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells.

ncbi gi num :

1352515

ncbi acc num :

P48745.1

uniprot acc num :

P48745

ncbi mol weight :

39,162 Da

ncbi pathways :

Delta-Notch Signaling Pathway (198879)

ncbi summary :

The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq, Feb 2009]

uniprot summary :

NOV: Immediate-early protein likely to play a role in cell growth regulation. Belongs to the CCN family. Expression is down-regulated by WT1. Interacts with FBLN1. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 8q24.1. Cellular Component: proteinaceous extracellular matrix; cell soma; intracellular membrane-bound organelle; axon; dendrite. Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding; growth factor activity. Biological Process: cell-cell signaling; regulation of gene expression; regulation of cell growth; cell adhesion; signal transduction

size1 :

96-Strip-Wells

price1 :

425 USD

more info or order :

MyBioSource product webpage