Mouse CRP PicoKine ELISA Kit | MyBioSource MBS177325 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse CRP PicoKine ELISA Kit

catalog :

MBS177325

quantity :

96-Strip-Wells

price :

425 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS177325

products type :

ELISA Kit

products full name :

Mouse CRP PicoKine ELISA Kit

products short name :

[CRP]

products name syn :

[C-reactive protein; C-reactive protein, pentraxin-related; C reactive protein pentraxin related; C-reactive protein; C-reactive protein(1-205); CRP; CRP_HUMAN; MGC149895; MGC88244; Pentraxin 1; Pentraxin 1 short; PTX 1; PTX1]

other names :

[C-reactive protein; C-reactive protein; C-reactive protein; C-reactive protein, petaxin related; C-reactive protein, pentraxin-related]

products gene name :

[CRP]

other gene names :

[Crp; Crp; AI255847; Ptx1]

uniprot entry name :

CRP_MOUSE

reactivity :

Mouse

sequence length :

225

specificity :

Natural and recombinant Mouse Crlf2

storage stability :

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta). Detection Range: 125pg/ml-8000pg/ml. Sensitivity: <10pg/ml

other info2 :

Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

products description :

Principle of the Assay: The Picokine Mouse Crlf2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Crlf2 with a 96-well strip plate that is pre-coated with antibody specific for Crlf2. The detection antibody is a biotinylated antibody specific for Crlf2. The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Mouse Crlf2 with immunogen: Expression system for standard: NSO, Immunogen sequence: A20-L233. The kit is analytically validated with ready to use reagents. To measure Mouse Crlf2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Mouse Crlf2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Crlf2 in the sample!!Background/Introduction: Cytokine receptor-like factor 2, also known as TSLPR or CRL2, is a protein that in humans is encoded by the CRLF2 gene. It is mapped to Xp22.33. The protein encoded by this gene is a receptor for thymic stromal lymphopoietin(TSLP). Together with the interleukin 7 receptor(IL7R), the encoded protein and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system. Two transcript variants encoding different isoforms have been found for this gene. In addition to it, it has been found that rearrangement of CRLF2 and JAK mutation together contribute to leukemogenesis in B-progenitor ALL.

ncbi gi num :

408360035

ncbi acc num :

P14847.2

uniprot acc num :

P14847

ncbi mol weight :

25,360 Da

ncbi pathways :

Classical Antibody-mediated Complement Activation Pathway (1001242); Complement Cascade Pathway (1001237); Creation Of C4 And C2 Activators Pathway (1001239); Immune System Pathway (971216); Initial Triggering Of Complement Pathway (1001238); Innate Immune System Pathway (971212)

uniprot summary :

CRP: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Belongs to the pentaxin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; growth cone; extracellular region; filopodium. Molecular Function: protein homodimerization activity; low-density lipoprotein receptor binding; metal ion binding; complement component C1q binding; low-density lipoprotein binding; cholesterol binding; calcium ion binding. Biological Process: protein polymerization; positive regulation of nitric oxide biosynthetic process; negative regulation of vasodilation; wound healing; positive regulation of superoxide release; acute-phase response; negative regulation of superoxide release; complement activation, classical pathway

size1 :

96-Strip-Wells

price1 :

425 USD

more info or order :

MyBioSource product webpage