ELISA/assay

Mouse SAA PicoKine ELISA Kit

MBS177310

96-Strip-Wells

390 USD

ELISA Kit

Mouse SAA PicoKine ELISA Kit

[SAA]

[Serum amyloid A protein; serum amyloid A1; Amyloid fibril protein AA; Amyloid protein A; MGC111216; OTTMUSP00000045357; PIG4; SAA; SAA1; Serum amyloid A 1; Serum amyloid A 1 protein; serum amyloid A protein; Serum amyloid A protein precursor; Serum amyloid A1 isoform 1; Serum amyloid A1 isoform 2; TP53I4; Tumor protein p53 inducible protein 4]

[SAA1]

Mouse

Natural and recombinant Mouse SAA/SAA1

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma(heparin, EDTA) and urine. Assay Type: Quantitative Sandwich. Detection Range: 1.56ng/ml-100ng/ml. Sensitivity: <150pg/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Principle of the Assay: The Mouse SAA/SAA1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SAA/SAA1 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: G20-Y122) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAA/SAA1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse SAA/SAA1 amount of sample captured in plate. Background/Introduction: Serum amyloid A protein(SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.

96-Strip-Wells

390 USD