Human Elafin/PI3 PicoKine ELISA Kit | MyBioSource MBS177123 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Elafin/PI3 PicoKine ELISA Kit

catalog :

MBS177123

quantity :

96 Wells

price :

425 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS177123

products type :

ELISA Kit

products full name :

Human Elafin/PI3 PicoKine ELISA Kit

products short name :

Elafin/PI3

products name syn :

Elafin; peptidase inhibitor 3, skin-derived; Cementoin; Elafin; Elastase specific inhibitor; ESI; MGC13613; Peptidase inhibitor 3; Peptidase inhibitor 3 skin derived; PI 3; PI3; Pre elafin; Protease inhibitor WAP3; SKALP; Skin derived antileukoproteinase; Trappin 2; WAP four disulfide core domain 14; WAP four disulfide core domain protein 14; WAP3; WFDC14

other names :

Elafin; Elafin; elafin; PI-3; trappin-2; pre-elafin; protease inhibitor WAP3; elastase-specific inhibitor; skin-derived antileukoproteinase; WAP four-disulfide core domain 14; WAP four-disulfide core domain protein 14; protease inhibitor 3, skin-derived (SKALP); peptidase inhibitor 3, skin-derived; Elastase-specific inhibitor; ESI; Peptidase inhibitor 3; PI-3; Protease inhibitor WAP3; Skin-derived antileukoproteinase; SKALP; WAP four-disulfide core domain protein 14

products gene name :

PI3

other gene names :

PI3; PI3; ESI; WAP3; SKALP; WFDC14; cementoin; WAP3; WFDC14; ESI; PI-3; SKALP

uniprot entry name :

ELAF_HUMAN

reactivity :

Human

sequence length :

117

specificity :

Natural and recombinant human Elafin

storage stability :

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

other info1 :

Samples: Cell culture supernates, serum and plasma (heparin, EDTA). Assay Type: Sandwich. Detection Range: 31.2pg/ml-2000pg/ml. Sensitivity: < 10pg/ml

other info2 :

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

products description :

Principle of the assay: human Elafin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Elafin has been precoated onto 96-well plates. Standards(NSO, A23-Q117) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Elafin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Elafin amount of sample captured in plate. Background: Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase (SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13.12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

ncbi gi num :

119262

ncbi acc num :

P19957.3

uniprot acc num :

P19957

ncbi mol weight :

12,270 Da

ncbi summary :

This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. [provided by RefSeq, Oct 2008]

uniprot summary :

elafin: Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. Protein type: Extracellular matrix; Inhibitor; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: proteinaceous extracellular matrix. Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity. Biological Process: copulation

size1 :

96 Wells

price1 :

425 USD

more info or order :

MyBioSource product webpage