ELISA/assay

Human ESM1/Endocan PicoKine ELISA Kit

MBS177114

96 Wells

390 USD

ELISA Kit

Human ESM1/Endocan PicoKine ELISA Kit

ESM1/Endocan

Endothelial cell-specific molecule 1; endothelial cell-specific molecule 1; Endocan; Endothelial cell specific molecule 1; Endothelial cell-specific molecule 1; ESM 1; ESM 1 secretory protein; ESM-1; Esm1; ESM1 secretory protein; ESM1_HUMAN

Endothelial cell-specific molecule 1; Endothelial cell-specific molecule 1; endothelial cell-specific molecule 1; ESM-1; endothelial cell-specific molecule 1

ESM1

ESM1; ESM1; endocan; ESM-1

ESM1_HUMAN

Human

184

Natural and recombinant human ESM1

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates, serum and plasma (heparin, EDTA). Assay Type: Sandwich. Detection Range: 31.2pg/ml-2000pg/ml. Sensitivity: < 10pg/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Principle of the assay: human ESM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ESM1 has been precoated onto 96-well plates. Standards(NSO, W20-R184) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ESM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ESM1 amount of sample captured in plate. Background: Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF(vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2).

13431509

Q9NQ30.2

Q9NQ30

14,513 Da

Gastric Cancer Network 1 Pathway (760635)

This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

ESM1: May have potent implications in lung endothelial cell- leukocyte interactions. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 5q11.2. Cellular Component: extracellular region. Molecular Function: integrin binding; insulin-like growth factor binding; hepatocyte growth factor receptor binding. Biological Process: positive regulation of cell proliferation; sprouting angiogenesis; regulation of cell growth; angiogenesis

96 Wells

390 USD