antibody

Anti-FLI1 antibody

MBS177100

0.1 mg

315 USD

polyclonal

rabbit

nonconjugated

human, rat

western blot, immunohistochemistry

Antibody

Anti-FLI1 antibody

[FLI1]

[Friend leukemia integration 1 transcription factor; Friend leukemia virus integration 1; ERGB transcription factor antibody; Ewing Sarcoma breakpoint region 2 antibody; EWSR 2 antibody; EWSR2 antibody; FLI 1 antibody; FLI 1 proto oncogene antibody; FLI1 antibody; FLI1 EWS fusion gene antibody; FLI1 proto oncogene antibody; FLI1_HUMAN antibody; Friend leukemia integration 1 transcription factor antibody; Friend leukemia virus integration 1 antibody; Proto-oncogene Fli-1 antibody; SIC 1 antibody; SIC1 antibody; Transcription factor ERGB antibody; Viral integration region FLI1 antibody]

[Friend leukemia integration 1 transcription factor; Friend leukemia integration 1 transcription factor; Friend leukemia integration 1 transcription factor; transcription factor ERGB; Ewing sarcoma breakpoint region 2; Friend leukemia virus integration 1; Fli-1 proto-oncogene, ETS transcription factor; Proto-oncogene Fli-1; Transcription factor ERGB]

[FLI1]

[FLI1; FLI1; EWSR2; SIC-1]

FLI1_HUMAN

Polyclonal

IgG

Rabbit

Reacts with: Human, Rat. Predicted to work with: Mouse

452

No cross reactivity with other proteins

Immunogen affinity purified.

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

At -20°C for one year. After reconstitution, at 4° C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB), Immunohistochemistry (Paraffin-embedded Section)

Western Blot: . Concentration: 0.1-0.5 ug/ml. Tested Species: Human. Predicted Species: Mouse, Rat. Antigen Retrieval: N/A. Immunohistochemistry (Paraffin-embedded Section): . Concentration: 0.5-1 ug/ml. Tested Species: Human, Rat. Predicted Species: Mouse. Antigen Retrieval: By Heat. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the parrafin sections in 10mM citrate buffer, pH 6.0 for 20 mins is required for the staining of formalin/paraffin sections. Other applications ahave not been tested/Optimal dilutions should be determined by end users.

Immunohistochemistry (IHC)

Western Blot (WB)

Immunohistochemistry (IHC)

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human FLI1 (54-73aa QQEWINQPVRVNVKREYDHM), identical to the related rat and mouse sequences.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Description: Rabbit IgG polyclonal antibody for Friend leukemia integration 1 transcription factor(FLI1) detection. Tested with WB, IHC-P in Human, Mouse, Rat. Background: Friend leukemia integration 1 transcription factor, also known as Transcription factor ERGB or Proto-oncogene Fli-1 is a protein that in humans is encoded by the human FLI1 gene. The fli1 protooncogene encodes a member of the ETS family of winged helix-turn-helix transcription factors. This gene is mapped to 11q24.3. Fli-1 is activated through retroviral insertional mutagenesis in 90% of F-MuLV-induced erythroleukemias. The constitutive activation of fli-1 in erythroblasts leads to a dramatic shift in the Epo/Epo-R signal transduction pathway, blocking erythroid differentiation, activating the Ras pathway, and resulting in massive Epo-independent proliferation of erythroblasts. These results suggest that Fli-1 overexpression in erythroblasts alters their responsiveness to Epo and triggers abnormal proliferation by switching the signaling event(s) associated with terminal differentiation to proliferation.

399496

Q01543.1

Q01543

29,084 Da

Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

FLI1: Sequence-specific transcriptional activator. Recognizes the DNA sequence 5 -C[CA]GGAAGT-3 . Defects in FLI1 are a cause of Ewing sarcoma (ES). A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription factor; Oncoprotein; DNA-binding. Chromosomal Location of Human Ortholog: 11q24.1-q24.3. Cellular Component: nucleus. Molecular Function: DNA binding; chromatin binding; transcription factor activity. Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; organ morphogenesis; hemostasis; blood circulation; positive regulation of transcription from RNA polymerase II promoter; cell differentiation

0.1 mg

315 USD