antibody

Anti-Adiponectin antibody

MBS176940

0.1 mg

280 USD

polyclonal

rabbit

nonconjugated

mouse, rat

western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Anti-Adiponectin antibody

Adiponectin

Adiponectin; adiponectin, C1Q and collagen domain containing; 30 kDa adipocyte complement related protein antibody; 30 kDa adipocyte complement-related protein antibody; ACDC antibody; ACRP 30 antibody; ACRP30 antibody; ADIPO_HUMAN antibody; Adipocyte antibody; Adipocyte C1q and collagen domain containing protein antibody; Adipocyte complement related 30 kDa protein antibody; Adipocyte complement related protein of 30 kDa antibody; Adipocyte complement-related 30 kDa protein antibody; Adiponectin antibody; AdipoQ antibody; Adipose most abundant gene transcript 1 antibody; Adipose most abundant gene transcript 1 protein antibody; Adipose specific collagen like factor antibody; ADIPQTL1 antibody; ADPN antibody; APM 1 antibody; apM-1 antibody; ApM1 antibody; C1q and collagen domain-containing protein antibody; GBP 28 antibody; GBP28 antibody; Gelatin binding protein antibody; Gelatin binding protein 28 antibody; Gelatin-binding protein antibody

30 kDa adipocyte complement-related protein (Adiponectin, C1Q and collagen domain containing); 30 kDa adipocyte complement-related protein; adiponectin; adipocyte complement related protein of 30 kDa; adiponectin, C1Q and collagen domain containing; 30 kDa adipocyte complement-related protein

ADIPOQ

Adipoq; Adipoq; Acdc; Acrp30

Q8K3R4_RAT

Polyclonal

IgG

Rabbit

Mouse, Rat

244

No cross reactivity with other proteins.

Immunogen affinity purified.

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot (WB):. Concentration: 0.1-0.5ug/ml. Tested Species: Rat. Antigen Retrieval: -. Immunohistochemistry (Paraffin-embedded Section): . Concentration: 0.5-1 ug/ml. Tested Species: Ms, Rat. Antigen Retrieval: By Heat. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/ paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen: E Coli-derived rat Adiponectin recombinant protein (Position: I19-N244). Rat Adiponectin shares 90% amino acid (aa) sequence identity with mouse Adiponectin.

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Description: Rabbit IgG polyclonal antibody for Adiponectin(ADIPOQ) detection. Tested with WB, IHC-P in Mouse, Rat. Background: Adiponectin, also known as APM1 or ACDC, is a protein which in humans is encoded by the ADIPOQ gene. It is mapped to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, it has been showed that Adiponectin is expressed by differentiated adipocytes as a 33-kD protein that is also detectable in serum. By sequence comparisons, it has been found that links between APM1 and TNF family ligands as well as to cytokines expressed by T cells. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. This gene is exclusively secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones.

81901098

Q8K3R4

Q8K3R4

26,410 Da

AMPK Signaling Pathway (989832); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (83485); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198479); Developmental Biology Pathway (1009870); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862252); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862314); PPAR Signaling Pathway (83434); PPAR Signaling Pathway (450)

adipocyte complement-related factor that plays a role in obesity-related insulin resistance [RGD, Feb 2006]

adiponectin: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2. Interacts with CTRP9A via the C1q domain (heterotrimeric complex). Synthesized exclusively by adipocytes and secreted into plasma. Protein type: Hormone; Secreted; Secreted, signal peptide; Endoplasmic reticulum. Cellular Component: extracellular space; protein complex; collagen; cell surface; endoplasmic reticulum; perinuclear region of cytoplasm. Molecular Function: identical protein binding; protein homodimerization activity; hormone activity; sialic acid binding; receptor binding. Biological Process: circadian rhythm; negative regulation of MAP kinase activity; negative regulation of phagocytosis; negative regulation of smooth muscle cell proliferation; negative regulation of hormone secretion; membrane hyperpolarization; positive regulation of cellular protein metabolic process; response to glucocorticoid stimulus; negative regulation of smooth muscle cell migration; glucose homeostasis; negative regulation of granulocyte differentiation; positive regulation of interleukin-8 production; positive regulation of glucose import; negative regulation of gluconeogenesis; response to glucose stimulus; adiponectin-mediated signaling pathway; negative regulation of protein amino acid autophosphorylation; negative regulation of blood pressure; negative regulation of cell migration; protein homooligomerization; response to nutrient; response to nutrient levels; response to drug; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of signal transduction; negative regulation of heterotypic cell-cell adhesion; glucose metabolic process; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of tumor necrosis factor production; negative regulation of fat cell differentiation; negative regulation of synaptic transmission; response to sucrose stimulus; membrane depolarization; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; fatty acid beta-oxidation; positive regulation of fatty acid metabolic process; negative regulation of low-density lipoprotein receptor biosynthetic process; negative regulation of macrophage differentiation; cellular response to insulin stimulus; negative regulation of inflammatory response; positive regulation of protein kinase activity; brown fat cell differentiation; response to hypoxia; fatty acid oxidation; response to activity; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; positive regulation of myeloid cell apoptosis; positive regulation of blood pressure

0.1 mg

280 USD