Anti-CMA1 Antibody | MyBioSource MBS176889 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-CMA1 Antibody

catalog :

MBS176889

quantity :

0.1 mg

price :

315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-CMA1 Picoband antibody, MBS176889. IHC(P): Human Placenta Tissue

image 2 :

Western blot analysis of CMA1 using anti- CMA1 antibody (MBS176889). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates,. Lane 2: human HepG2 whole cell lysates,. Lane 3: rat liver tissue lysates,. Lane 4: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CMA1 antigen affinity purified polyclonal antibody (Catalog # MBS176889) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for CMA1 at approximately 35KD. The expected band size for CMA1 is at 27KD.

product information

catalog number :

MBS176889

products type :

Antibody

products full name :

Anti-CMA1 Antibody

products short name :

[CMA1]

products name syn :

[Chymase; chymase 1, mast cell; Alpha-chymase antibody; Chymase 1 antibody; Chymase 1 mast cell antibody; Chymase antibody; Chymase, heart antibody; Chymase, mast cell antibody; CMA1 antibody; CMA1_HUMAN antibody; CYH antibody; CYM antibody; EC 3.4.21.39 antibody; Mast cell chymase 1 antibody; Mast cell protease 3 antibody; Mast cell protease 5 antibody; Mast cell protease I antibody; Mast cell protease III antibody; Mcp-5 antibody; MCP3P antibody; Mcpt5 antibody; MGC119890 antibody; MGC119891 antibody; MMCP-5 antibody]

other names :

[Chymase; Chymase; chymase; alpha-chymase; chymase, heart; chymase, mast cell; mast cell protease I; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; chymase 1, mast cell; Alpha-chymase; Mast cell protease I]

products gene name :

[CMA1]

other gene names :

[CMA1; CMA1; CYH; MCT1; chymase; CYH; CYM]

uniprot entry name :

CMA1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Reacts with Human. No cross reactivity with other proteins

sequence length :

247

purity :

Immunogen affinity purified.

form :

Lyophilized

storage stability :

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

app notes :

Western Blot:. Concentration: 0.1-0.5 ug/ml. Tested Species: Hu. IHC (Paraffin-embedded Section):. Concentration: 0.5-1 ug/ml. Tested Species: Hu. Antigen Retrieval: By Heat. WB: The detection limit for CMA1 is approximatelt 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications not tested. Optimal dilutions should be determined by the end users.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

other info1 :

Immunogen: E Coli-derived human CMA1 recombinant protein (Position: I22-N247). Human CMA1 shares 75% and 74% amino acid (aa) sequence identity with mouse and rat CMA1, respectively. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg NaN 3 . Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

products description :

Description: Rabbit IgG polyclonal antibody for Chymase(CMA1) detection. Tested with WB, IHC-P in Human. Background: Chymase is a major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. Chymase is mapped to 14q12. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II.

ncbi gi num :

126825

ncbi acc num :

P23946.1

uniprot acc num :

P23946

ncbi pathways :

ACE Inhibitor Pathway (198763); Activation Of Matrix Metalloproteinases Pathway (576264); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Metabolism Of Angiotensinogen To Angiotensins Pathway (685555); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Renin-angiotensin System Pathway (83075); Renin-angiotensin System Pathway (486); Signal Transduction Pathway (477114)

ncbi summary :

This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Angiotensin II has been implicated in blood pressure control and in the pathogenesis of hypertension, cardiac hypertrophy, and heart failure. Thus, this gene product is a target for cardiovascular disease therapies. This gene maps to 14q11.2 in a cluster of genes encoding other proteases. [provided by RefSeq, Jul 2008]

uniprot summary :

CMA1: Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. Belongs to the peptidase S1 family. Granzyme subfamily. Protein type: Secreted; Secreted, signal peptide; EC 3.4.21.39; Protease. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: extracellular matrix; extracellular space; extracellular region; intracellular. Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity; peptide binding. Biological Process: interleukin-1 beta biosynthetic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; positive regulation of angiogenesis; cellular protein metabolic process; regulation of inflammatory response; midbrain development; protein processing; proteolysis; angiotensin maturation

size1 :

0.1 mg

price1 :

315 USD

more info or order :

MyBioSource product webpage