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SDS-PAGE Protein Loading Buffer 5X (Reducing)

MBS176755

3 mL

80 USD

Western Blot Related Reagent

SDS-PAGE Protein Loading Buffer 5X (Reducing)

[SDS-PAGE Loading Buffer (5x)]

Liquid; 10% SDS, 500mM DTT, 50% Glycerol, 250mM Tris-HCL and 0.5% bromophenol blue dye, PH6.8.

Upon receipt store at -20°C. RIPA Lysis Buffer is stable for one year. Product is shipped on ice.

Equivalent: Please inquire.

Assay Principle: Using bromophenol blue dye, SDS-PAGE Protein Loading Buffer is a ready-to-use 5X solution. It contains 10% SDS, 500Mm DTT, 50% Glycerol, 500mM Tris-HCL and 0.05% bromophenol blue dye. It can be used for SDS-PAGE protein loading of conventional proteins. It is sufficient to prepare 12mL protein samples. And the whole sample preparation process can be finished within 5 minutes. Assay Protocol: 1. Equilibrate SDS-PAGE Protein Loading Buffer 5X to room temperature or thaw Loading Buffer in a water bath no higher than 30°C. 2. Mix one volume of SDS-PAGE Protein Loading Buffer 5X with four volume of protein sample (i.e. add 4mL protein sample into 1 mL Loading Buffer). 3. Boil sample for 3-5 min. Note: If semitransparent viscous substance remains after boiling, boil sample for another 5-10 min or add 1X SDS-PAGE Protein Loading Buffer which is diluted from 5X SDS-PAGE Protein Loading Buffer to the sample and then boil for another 3-5 min. 4. Allow sample to cool to room temperature. 5. Load sample into the wells of the SDS-PAGE gel and begin electrophoresis. 6. Stop electrophoresis when bromophenol blue dye front reaches to the bottom of the gel. General Notes: 1. SDS-PAGE Protein Loading Buffer 5X contains DTT which has a slightly irritating odor. 2. SDS-PAGE Protein Loading Buffer 5X should be completely dissolved before use. 3. Please wear the lab coat and disposable gloves and operate in a laboratory hood.

Western Blotting Related Reagent

This SDS PAGE Sample Buffer 5X (Reducing) is the most commonly used sample buffer for Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) of denatured proteins in the Laemmli SDS-PAGE system.

3 mL

80 USD