product summary
Loading...
company name :
MyBioSource
product type :
secondary antibody
product name :
5 nm Colloidal Gold Conjugated Goat Anti-mouse IgG secondary antibody
catalog :
MBS176697
quantity :
1 mL
price :
180 USD
more info or order :
product information
catalog number :
MBS176697
products type :
Secondary Antibody
products full name :
5 nm Colloidal Gold Conjugated Goat Anti-mouse IgG secondary antibody
products short name :
5 nm Colloidal Gold Conjugated Goat Anti-mouse IgG
clonality :
Polyclonal
isotype :
IgG
host :
Goat
specificity :
This colloidal gold conjugated antibody is specific for mouse IgG and shows no cross-reactivity with human, bovine, rabbit IgG.
form :
Concentrated, Liquid
storage stability :
At 4 degree C for one year.
tested application :
Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC), EM
app notes :
Recommended Dilutions. EM: 1:20-50. IHC-P (paraffin-fixed section): 1:50-100. IHC-F (frozen-fixed section): 1:50-100. ICC: 1:50-100. Optimal working dilutions must be determined by end users.
other info1 :
Immunogen: Mouse IgG (whole molecule).
other info2 :
Contents: 0.1 mg of colloidal gold conjugated specific antibody, the diameter of colloidal gold is 5±1 nm; 0.01 M PBS; 0.01 % Thimerosal, 50% glycerol. Preparation of Colloidal Gold: 5 nm Colloidal Gold - Add 0.05% trisodium citrate and 0.05% Vitamin C into boiled 0.01 % chloroauric acid which was reduced to gold granule solution through redox reaction. Conjugate colloidal gold to antibody after rnensurating the optimal data. And through overspeed centrifugation and gradient delamination to density, the colloidal gold conjugated reagents were produced. Preparation of Diluent Buffer: Add reagent grade BSA into 0.01 M PBS (PH7.2-7.6) or TBS buffer and make BSA at a concentration of 1%. Use the above diluent buffer to dilute. See "Recommended Dilutions" below for details. Preparation of IGSS (ImmunoGold Silver Staining): Add citric acid 2.35g, trisodium citrate 2.55g, glutin 19 and hydroquinone 1.7g into 100 ml of distilled water and mix evenly. Before usage, add silver nitrate 50mg into it. Then, add this mixed solution into tissue at last and colorate for 10-20 minutes in dark. Wash with water to terminate reaction .
products description :
Description: This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins and bovine serum proteins to ensure minimal cross reactivity in tissue or cell preparations.
size1 :
1 mL
price1 :
180 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!