product summary
Loading...
company name :
MyBioSource
product type :
protein
product name :
HRP Conjugated Streptavidin
catalog :
MBS176633
quantity :
0.5 mL
price :
160 USD
more info or order :
product information
catalog number :
MBS176633
products type :
Protein
products full name :
HRP Conjugated Streptavidin
products short name :
[HRP Conjugated Streptavidin]
form :
Concentrated, Liquid
concentration :
1mg/ml
storage stability :
At 4 degree C for one year.
tested application :
Dot Blot (DB), Western Blot (WB), ELISA (EIA)
app notes :
Dot blot(ECM): 0.25-0.5ug/ml. Western blot(DAB): 0.3-2ug/ml. Western blot(ECM): 0.1-0.3ug/ml. ELISA: 0.025-0.05ug/ml. Optimal working dilutions should be determined be end users.
other info1 :
Contents: 1 mg of peroxidase conjugated streptavidin; 0.01M PBS (pH 7.4), 50% glycerol. Labeling Method: Streptavidin is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK. Preparation of Dilutent Buffer: Use 0.01 M TBS or 0.01 M PBS to dilute:. Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450?l of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na 2 HPO 4 and 0.2g Na 2 HPO 4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
products description :
Description: Streptavidin is a 47KD protein that extracted from streptomyces. Just like avidin, streptavidin has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. Isoelectric point of the streptavidin is near to neutrality, IP=6.0~6.5. Thus, streptavidin has very low non-specific absorption to tissue and cell. On the basis of streptavidin, the background of immunohistochemistry analysis is extremely low. With the method of polymeric labeling, streptavidin-HRP can form a complex which composed by about one hundred HRP and fifty streptavidin. And lots of enzymes can ensure the streptavidin-HRP with high sensitivity.
size1 :
0.5 mL
price1 :
160 USD
size2 :
1 mL
price2 :
230
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!