ELISA/assay

Human CCL18/PARC PicoKine ELISA Kit

MBS176563

96 Wells

425 USD

ELISA Kit

Human CCL18/PARC PicoKine ELISA Kit

CCL18/PARC

C-C motif chemokine 18; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); Alternative macrophage activation associated CC chemokine 1; Alternative macrophage activation-associated CC chemokine 1; AMAC 1; AMAC-1; AMAC1; C C motif chemokine 18; CC chemokine ligand 18; CC chemokine PARC; CCL18; CCL18(4-69); CCL18_HUMAN; Chemokine (C C motif) ligand 18; Chemokine (C C) dendritic; Chemokine ligand 18; CKb7; DC CK1; DC-CK1; DCCK1; Dendritic cell chemokine 1; Macrophage inflammatory protein 4; MIP 4; MIP-4; MIP4; PARC; Pulmonary and activation regulated chemokine; Pulmonary and activation-regulated chemokine; Small inducible cytokine A18; Small-inducible cytokine A18

C-C motif chemokine 18; C-C motif chemokine 18; C-C motif chemokine 18; CC chemokine PARC; CC chemokine ligand 18; chemokine (C-C), dendritic; dendritic cell chemokine 1; small inducible cytokine A18; small-inducible cytokine A18; macrophage inflammatory protein 4; pulmonary and activation-regulated chemokine; alternative macrophage activation-associated CC chemokine 1; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary and activation-regulated; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); Alternative macrophage activation-associated CC chemokine 1; AMAC-1; CC chemokine PARC; Dendritic cell chemokine 1; DC-CK1; Macrophage inflammatory protein 4; MIP-4; Pulmonary and activation-regulated chemokine; Small-inducible cytokine A18Cleaved into the following 3 chains:CCL18(1-68); CCL18(3-69); CCL18(4-69)

CCL18

CCL18; CCL18; CKb7; PARC; AMAC1; DCCK1; MIP-4; AMAC-1; DC-CK1; SCYA18; AMAC1; DCCK1; MIP4; PARC; SCYA18; AMAC-1; DC-CK1; MIP-4

CCL18_HUMAN

Human

89

Natural and recombinant human CCL18

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates and serum. Assay Type: Sandwich. Detection Range: 7.8pg/ml-500pg/ml. Sensitivity: < 10pg/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Principle of the assay: human CCL18 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL18 has been precoated onto 96-well plates. Standards(E.coli, A21-A89) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL18 amount of sample captured in plate. Background: Chemokine (C-C motif) ligand 18 (CCL18) is a small cytokine belonging to the CC chemokine family that was previously called PARC (pulmonary and activation-regulated chemokine). CCL18 is approximately 60% identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma.

2493666

P55774.1

P55774

9,849 Da

Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460)

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for naive T cells, CD4+ and CD8+ T cells and nonactivated lymphocytes, but not for monocytes or granulocytes. This chemokine attracts naive T lymphocytes toward dendritic cells and activated macrophages in lymph nodes. It may play a role in both humoral and cell-mediated immunity responses. [provided by RefSeq, Sep 2014]

CCL18: Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses. Belongs to the intercrine beta (chemokine CC) family. Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Chemokine. Chromosomal Location of Human Ortholog: 17q12. Cellular Component: extracellular space. Molecular Function: protein binding; chemokine activity. Biological Process: cell-cell signaling; response to biotic stimulus; cell communication; immune response; signal transduction; inflammatory response; chemotaxis

96 Wells

425 USD