ELISA/assay

Human CXCL11/I-TAC PicoKine ELISA Kit

MBS176544

96 Wells

390 USD

ELISA Kit

Human CXCL11/I-TAC PicoKine ELISA Kit

CXCL11/I-TAC

C-X-C motif chemokine 11; chemokine (C-X-C motif) ligand 11; b R1; Beta R1; Beta-R1; betaR1; C X C motif chemokine 11; C-X-C motif chemokine 11; Chemokine (C-X-C motif) ligand 11; Chemokine C-X-C motif ligand 11; CXCL11; CXL11_HUMAN; H174; I TAC; I-TAC; Interferon gamma inducible protein 9; Interferon gamma-inducible protein 9; Interferon inducible T cell a chemoattractant I TAC; Interferon inducible T cell alpha chemoattractant; Interferon-inducible T-cell alpha chemoattractant; IP 9; IP-9; IP9; ITAC; MGC102770; SCYB11; SCYB9B; Small inducible cytokine B11; Small inducible cytokine subfamily B (Cys X Cys) member 11; Small inducible cytokine subfamily B (Cys X Cys) member 9B; Small inducible cytokine subfamily B, member 11; Small inducible cytokine subfamily B, member 9B; Small-inducible cytokine B11

C-X-C motif chemokine 11; C-X-C motif chemokine 11; C-X-C motif chemokine 11; beta-R1; small inducible cytokine B11; small-inducible cytokine B11; interferon gamma-inducible protein 9; interferon-inducible T-cell alpha chemoattractant; small inducible cytokine subfamily B (Cys-X-Cys), member 11; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; chemokine (C-X-C motif) ligand 11; Beta-R1; H174; Interferon gamma-inducible protein 9; IP-9; Interferon-inducible T-cell alpha chemoattractant; I-TAC; Small-inducible cytokine B11

CXCL11

CXCL11; CXCL11; IP9; H174; IP-9; b-R1; I-TAC; SCYB11; SCYB9B; ITAC; SCYB11; SCYB9B; IP-9; I-TAC

CXL11_HUMAN

Human

94

Natural and recombinant human CXCL11

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates, serum and plasma (heparin, EDTA). Assay Type: Sandwich. Detection Range: 62.5pg/ml-4000pg/ml. Sensitivity: < 10pg/ml

Principle of the assay: human CXCL11 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL11 has been precoated onto 96-well plates. Standards (E.coli,F22-F94) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL11 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL11 amount of sample captured in plate. Background: Chemokine (C-X-C motif) ligand 11 (CXCL11) is a small cytokine belonging to the CXC chemokine family that is also called Interferon-inducible T-cell alpha chemoattractant (I-TAC) and Interferon-gamma-inducible protein 9 (IP-9). By PCR analysis of somatic cell hybrids, the CXCL11 gene was mapped to chromosome 4, and using FISH, it was located to 4q21.2. The CXCL11 was strongly induced by IFNG and beta-interferon, but only weakly induced by alpha-interferon. Induction of CXCL11 required expression of STAT1. What's more, it found that monocyte chemotaxis occurs in response to recombinant CXCL11. RT-PCR analysis detected CXCL11 expression in brain tissue from a patient with AIDS dementia and a patient with multiple sclerosis but not in 2 controls.

7674360

O14625.1

O14625

10,365 Da

CXCR3-mediated Signaling Events Pathway (138011); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020)

Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC. This antimicrobial gene is a CXC member of the chemokine superfamily. Its encoded protein induces a chemotactic response in activated T-cells and is the dominant ligand for CXC receptor-3. The gene encoding this protein contains 4 exons and at least three polyadenylation signals which might reflect cell-specific regulation of expression. IFN-gamma is a potent inducer of transcription of this gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

CXCL11: Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses. Belongs to the intercrine alpha (chemokine CxC) family. Protein type: Secreted; Chemokine; Secreted, signal peptide; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 4q21.2. Cellular Component: extracellular space; extracellular region. Molecular Function: heparin binding; protein binding; CXCR3 chemokine receptor binding; chemokine activity. Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; immune response; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of cAMP metabolic process; inflammatory response; signal transduction; chemotaxis; regulation of cell proliferation

96 Wells

390 USD