ELISA/assay

Human B7-1/CD80 PicoKine ELISA Kit

MBS176498

96-Strip-Wells

425 USD

ELISA Kit

Human B7-1/CD80 PicoKine ELISA Kit

[B7-1/CD80]

[T-lymphocyte activation antigen CD80; CD80 molecule; Activation B7-1 antigen; B7; BB1; CD80; CD80_HUMAN; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80]

[T-lymphocyte activation antigen CD80; T-lymphocyte activation antigen CD80; T-lymphocyte activation antigen CD80; activation B7-1 antigen; costimulatory factor CD80; CTLA-4 counter-receptor B7.1; B-lymphocyte activation antigen B7; costimulatory molecule variant IgV-CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 molecule; Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1; B7; CD_antigen: CD80]

[CD80]

[CD80; CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1; CD28LG; CD28LG1; LAB7; B7]

CD80_HUMAN

Human

288

Natural and recombinant Human CD300LF

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta). Detection Range: 31.2pg/ml-2000pg/ml. Sensitivity: <10pg/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Principle of the Assay: The Picokine Human CD300LF Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CD300LF with a 96-well strip plate that is pre-coated with antibody specific for CD300LF. The detection antibody is a biotinylated antibody specific for CD300LF. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human CD300LF with immunogen: Expression system for standard: E.coli, Immunogen sequence: T20-S156. The kit is analytically validated with ready to use reagents. To measure Human CD300LF, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human CD300LF in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD300LF in the sample!!Background/Introduction: CMRF35-like molecule 1 is a protein that in humans is encoded by the CD300LF gene. It is mapped to 17q25.1. This gene encodes a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. The encoded protein is an inhibitory receptor. Alternative splicing results in multiple transcript variants.

461606

P33681.1

P33681

18,761 Da

Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); CD28 Co-stimulation Pathway (119553); CD28 Dependent PI3K/Akt Signaling Pathway (119554); CD28 Dependent Vav1 Pathway (119555); CTLA4 Inhibitory Signaling Pathway (119556)

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]

CD80: Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. Protein type: Membrane protein, integral; Immunoglobulin superfamily. Chromosomal Location of Human Ortholog: 3q13.3-q21. Cellular Component: cell surface; plasma membrane; integral to membrane; external side of plasma membrane. Molecular Function: protein binding; coreceptor activity. Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; viral reproduction; T cell activation; positive regulation of signal transduction; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of interleukin-2 biosynthetic process; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell signaling; T cell costimulation; innate immune response; positive regulation of alpha-beta T cell proliferation; positive regulation of T-helper 1 cell differentiation

96-Strip-Wells

425 USD