catalog number :
MBS176451
products type :
Detection Kit
products full name :
HRP Conjugated anti-Rabbit IgG SABC Kit
products short name :
[HRP Conjugated anti-Rabbit IgG]
form :
Ready to use (No dilution needed)
storage stability :
4 degree C for one year. Avoid freezing
tested application :
Immunohistochemistry (IHC) Paraffin, IImmunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC)
image1 heading :
Immunohistochemistry (IHC)
image2 heading :
Immunohistochemistry (IHC)
products description :
Background: SABC (StreptAvidin-Biotin Complex) is specially designed for displaying the distribution of antigens in tissues and cells in immunochemistry and other immunodetection analyses. Streptavidin is a 47,000 dalton protein purified from the bacterium Streptomyces avidinii. Streptavidin has extraordinarily strong affinity to biotin molecules. The dissociation constant (Kd) of the biotin-streptavidin complex is on the order ~10-15 mol/L, a million times higher than the typical affinity between antigens and their antibodies. Streptavidin has very low non-specific binding to tissues and cells, due to its nearly neutral isoelectric point (IP=6.0~6.5). Therefore, immunohistochemical analyses based on streptavidin-biotin complex has extremely low background. Furthermore, this kit has high sensitivity because each complex it generates has a large number of alkaline phosphatase and streptavidin molecules. In brief, SABC offers high specificity, low background and ease-of-use.