Polyclonal Anti-CDH5 Antibody; Anti-VE Cadherin antibody | MyBioSource MBS176401 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Anti-CDH5 Antibody; Anti-VE Cadherin antibody

catalog :

MBS176401

quantity :

0.1 mg

price :

315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-VE Cadherin antibody, MBS176401, Western blotting. All lanes: Anti VE Cadherin (MBS176401) at 0.5ug/ml. Lane 1: A549 Whole Cell Lysate at 40ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: MCF-7 Whole Cell Lysate at 40ug. Predicted bind size: 87KD. Observed bind size: 130KD

image 2 :

Anti-VE Cadherin antibody, MBS176401, IHC(P). IHC(P): Human Lung Cancer Tissue

image 3 :

Anti-VE Cadherin antibody, MBS176401, IHC(P). IHC(P): Rat Brain Tissue

product information

catalog number :

MBS176401

products type :

Antibody

products full name :

Polyclonal Anti-CDH5 Antibody; Anti-VE Cadherin antibody

products short name :

[VE Cadherin]

products name syn :

[Cadherin-5; cadherin 5, type 2(vascular endothelium); 7B 4 antibody; 7B4 antibody; 7B4 antigen antibody; CADH5_HUMAN antibody; Cadherin 5 antibody; Cadherin 5 type 2 antibody; Cadherin 5, type 2(vascular endothelium) antibody; Cadherin 5, type 2, VE cadherin(vascular epithelium) antibody; cadherin, vascular endothelial antibody; cadherin, vascular endothelial, 1 antibody; Cadherin-5 antibody; Cadherin5 antibody; CD 144 antibody; CD144 antibody; CD144 antigen antibody; CDH 5 antibody; CDH5 antibody; CDH5 protein antibody; Endothelial specific cadherin antibody; FLJ17376 antibody; OTTHUMP00000174777 antibody; Vascular endothelial cadherin antibody; Vascular epithelium cadherin antibody; VE Cad antibody; VE-cadherin antibody; VEC antibody]

other names :

[Cadherin-5; Cadherin-5; cadherin-5; 7B4 antigen; VE-cadherin; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin; cadherin 5, type 2, VE-cadherin (vascular epithelium); cadherin 5, type 2 (vascular endothelium); 7B4 antigen; Vascular endothelial cadherin; VE-cadherin; CD_antigen: CD144]

products gene name :

[CDH5]

other gene names :

[CDH5; CDH5; 7B4; CD144; VE-cadherin]

uniprot entry name :

CADH5_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Rat; Predicted: Mouse

sequence length :

784

specificity :

No cross reactivity with other proteins

purity :

Immunogen affinity purified.

form :

Lyophilized

storage stability :

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

app notes :

Western Blot: . Concentration: 0.1-0.5ug/ml. Tested Species: Human. Predicted Species: N/A. Antigen retrieval: N/A. Immunohistochemistry (Paraffin-embedded Section): . Concentration: 0.5-1ug/ml. Tested Species: Human, Rat. Predicted Species: Mouse. Antigen retrieval: By Heat. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested, Optimal dilutions should be determined by end users.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CDH5(766-784aa RFKMLAELYGSDPREELLY), different from the related mouse and rat sequences by three amino acids.

other info2 :

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 . Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

products description :

CDH5(Cadherin 5), also known as VE-cadherin, is a type of cadherin. It is encoded by the human gene CDH5. Kremmidiotis et al.(1998) mapped the human CDH5 gene to 16q22.1 using somatic cell hybrid panels. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Therefore it was concluded that VE-cadherin serves the purpose of maintaining newly formed vessels.

ncbi gi num :

322510142

ncbi acc num :

P33151.5

uniprot acc num :

P33151

ncbi pathways :

Adherens Junctions Interactions Pathway (119533); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-cell Junction Organization Pathway (119532); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); S1P2 Pathway (138059); Signal Transduction Pathway (477114)

ncbi summary :

This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain. Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral. Chromosomal Location of Human Ortholog: 16q22.1. Cellular Component: adherens junction; tight junction; membrane; integral to membrane; plasma membrane; intercellular junction; cell junction. Molecular Function: protein binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; receptor binding. Biological Process: intercellular junction assembly and maintenance; negative regulation of cell proliferation; vascular endothelial growth factor receptor signaling pathway; homophilic cell adhesion; blood vessel maturation

size1 :

0.1 mg

price1 :

315 USD

more info or order :

MyBioSource product webpage