antibody

Anti-PC1/3 antibody

MBS176272

0.1 mg

245 USD

polyclonal

rabbit

nonconjugated

human, mouse

Antibody

Anti-PC1/3 antibody

PC1/3

Neuroendocrine convertase 1; proprotein convertase subtilisin/kexin type 1; BDP antibody; EC antibody; NEC 1 antibody; NEC1 antibody; NEC1_HUMAN antibody; Neuroendocrine convertase 1 antibody; PC1 antibody; PC3 antibody; PCSK1 antibody; Prohormone convertase 1 antibody; Prohormone convertase 1/3 antibody; Prohormone convertase 3 antibody; Proprotein convertase 1 antibody; SPC3 antibody

Neuroendocrine convertase 1; Neuroendocrine convertase 1; neuroendocrine convertase 1; prohormone convertase 1; prohormone convertase 3; proprotein convertase subtilisin/kexin type 1; Prohormone convertase 1; Proprotein convertase 1; PC1

PCSK1

PCSK1; PCSK1; PC1; PC3; NEC1; SPC3; BMIQ12; NEC1; NEC 1; PC1

NEC1_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat. No cross reactivity with other proteins.

753

Immunogen affinity purified.

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Other applications have not been tested.

Western Blot Concentration: 0.1-0.5ug/ml. Western Blot Tested Species: Human, Rat. Western Blot Concluded Species: Mouse. WB: The detection limit for PCSK1 is approximately 1ng/lane under reducing conditions. IHC-P Concentration: 0.5-1ug/ml. IHC-P Tested Species: Rat. IHC-P Concluded Species: Human, Mouse. IHC-P Antigen Retrievel: By Heat. Note: Tested Species: In-hose tested species with positive results. Concluded Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling paraffin section in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Optimal dilutions should be determined by end users.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PC1/3 (726-740aa DVFYNTKPYKHRDDR), identical to the related mouse and rat sequences. Relevant Detection Systems: MyBioSource provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit in WB.

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Description: Rabbit IgG polyclonal antibody for Neuroendocrine convertase 1(PCSK1) detection. Tested with WB, IHC-P in Human, Mouse, Rat. Background: PCSK1(Proprotein Convertase, Subtilisin/Kexin-Type, 1), also known as PC1 or NEC1, is an enzyme that in humans is encoded by the PCSK1 gene. Proprotein convertase-1 is a neuroendocrine convertase that belongs to a family of subtilisin-like serine endoproteases that process large precursor proteins into mature bioactive products. By in situ hybridization, Seidah et al.(1991) mapped the NEC1 gene to human chromosome 5q15-q21 and mouse chromosome 13. Ohagi et al.(1996) noted that PC1 initiates the sequential processing of proinsulin to insulin by cleaving the proinsulin molecule on the C-terminal side of the dibasic peptide, arg31-arg32, joining the B-chain and C-peptide. By observing the phenotypic features in patients with PC1 mutations, Jackson et al.(2003) concluded that human intestinal absorptive function is dependent on PC1 activity.

116242674

P29120.2

P29120

79,010 Da

Incretin Synthesis, Secretion, And Inactivation Pathway (187170); Insulin Processing Pathway (105903); Metabolism Of Proteins Pathway (106230); Peptide Hormone Biosynthesis Pathway (160988); Peptide Hormone Metabolism Pathway (771603); Synthesis, Secretion, And Deacylation Of Ghrelin Pathway (119538); Synthesis, Secretion, And Inactivation Of Glucagon-like Peptide-1 (GLP-1) Pathway (187171); Synthesis, Secretion, And Inactivation Of Glucose-dependent Insulinotropic Polypeptide (GIP) Pathway (187172)

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to subcellular compartments where a second autocatalytic even takes place and the catalytic activity is acquired. The protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Mutations in this gene have been associated with susceptibility to obesity and proprotein convertase 1/3 deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene [provided by RefSeq, Jan 2014]

PCSK1: Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin. Belongs to the peptidase S8 family. Furin subfamily. Protein type: EC 3.4.21.93; Protease. Chromosomal Location of Human Ortholog: 5q15-q21. Cellular Component: Golgi apparatus; extracellular space; transport vesicle. Molecular Function: serine-type endopeptidase activity. Biological Process: cellular protein metabolic process; cell-cell signaling; metabolic process; peptide hormone processing; proteolysis; regulation of insulin secretion; peptide biosynthetic process. Disease: Proprotein Convertase 1/3 Deficiency; Body Mass Index Quantitative Trait Locus 12

0.1 mg

245 USD