antibody

Anti-MEKK1 antibody

MBS176258

0.1 mg

315 USD

polyclonal

rabbit

nonconjugated

human, rat

western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Anti-MEKK1 antibody

[MEKK1]

[Mitogen-activated protein kinase kinase kinase 1; mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase; M3K1_HUMAN antibody; MAP3K1 antibody; MAPK/ERK kinase kinase 1 antibody; MAPKKK1 antibody; MEK kinase 1 antibody; MEKK 1 antibodyMekk antibody; MEKK1 antibody; Mitogen activated protein kinase kinase kinase 1 antibody; Mitogen activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase antibody; Mitogen-activated protein kinase kinase kinase 1 antibodySRXY6 antibody]

[Mitogen-activated protein kinase kinase kinase 1; Mitogen-activated protein kinase kinase kinase 1; mitogen-activated protein kinase kinase kinase 1; MEK kinase 1; MAP/ERK kinase kinase 1; MAPK/ERK kinase kinase 1; mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase; MAPK/ERK kinase kinase 1; MEK kinase 1; MEKK 1]

[MAP3K1]

[MAP3K1; MAP3K1; MEKK; MEKK1; SRXY6; MEKK 1; MAPKKK1; MAPKKK1; MEKK; MEKK1; MEK kinase 1; MEKK 1]

M3K1_HUMAN

Polyclonal

IgG

Rabbit

Reacts with: Human. Predicted: Mouse, Rat

1512

Immunogen affinity purified.

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot:. Concentration: 0.1-0.5ug/ml. Tested Species: Hu. Predicted Species: Ms, Rat. Immunohistochemistry (Paraffin-embedded Section):. Cocentration:0.5-1ug/ml. Tested Species: Hu. Predicted Species: Ms, Rat. Antigen Retrieval: By Heat. WB: The detection limit for MAP3K1 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of fomalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be detemined by end users.

Immunohistochemistry (IHC)

Western Blot (WB)

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MEKK1 (1418-1432aa PEVLRGQQYGRSCDV), identical to the related rat and mouse sequences.

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Description: Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase kinase kinase 1(MAP3K1) detection. Tested with WB, IHC-P in Human, Mouse, Rat. Background: MAP3K1(Mitogen-activated protein kinase kinase kinase 1), also known as MEKK1, MAPKKK1, MEK KINASE or MAP/ERK KINASE KINASE 1, is an enzyme that in humans is encoded by the MAP3K1 gene. Vinik et al.(1995) identified DNA sequence and size polymorphisms in intronic and 3-prime untranslated regions of the mouse Map3k1 gene and the human MAP3K1 homolog. Using these allele-specific polymorphisms, they mapped the Map3k1 gene in an intersubspecific backcross to mouse chromosome 13. They mapped the human MAP3K1 gene to chromosome 5 by somatic cell hybrid analysis. By assaying transfected COS-1 cells, Xia et al.(1998) showed that human MEKK1 activated JNK1(MAPK8) robustly and p38-alpha(MAPK14) less efficiently, but it had only a marginal effect on ERK2(MAPK1). MEKK1 directly and specifically interacted with JNKK1(MAP2K4) and activated JNKK1 in cells and in vitro. Phosphorylation of JNKK1 by MEKK1 disrupted their interaction. MEKK1 and JNK1 competed for binding to JNKK1. Xia et al.(1998) concluded that JNKK1 is the preferred MEKK1 substrate.

218512139

Q13233.4

Q13233

164,470 Da

Activated TLR4 Signalling Pathway (106400); Apoptosis Pathway (198797); Apoptosis Modulation By HSP70 Pathway (198877); BCR Signaling Pathway (138058); BDNF Signaling Pathway (712093); CD40/CD40L Signaling Pathway (138061); CDC42 Signaling Events Pathway (137994); Caspase Cascade In Apoptosis Pathway (137974); Ceramide Signaling Pathway (138023); DNA Damage Response (only ATM Dependent) Pathway (198827)

The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. [provided by RefSeq, Mar 2012]

MEKK1: a protein kinase of the STE11 family. Phosphorylates and activates MKK4 and -7, which in turn activate JNK1, -2 and -3. Contains 1 RING-type zinc finger. The RING finger domain of MEKK1 exhibits E3 ubiquitin ligase activity toward c-Jun. c-Jun is downregulated in response to osmotic stress via ubiquitination-mediated degradation by the RING finger domain of MEKK1. Overexpressed/overactivated in multiple tumor types. The Mek1/2 inhibitor U0126 blocks export of influenza viral particles and has been suggested as an antiviral treatment. Single mutations at the same point of each gene also associated with cardiofaciocutaneous syndrome, (also linked with Braf and Kras mutations), displaying morphological, cardiac and mental defects. Inhibitors: U0126, CI-1040/PD184352, PD-0325901 (Phase I cancer), ARRY-142886 (Phase 1, cancer). Protein type: Ubiquitin ligase; Ligase; EC 2.7.11.25; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Ubiquitin conjugating system; STE group; STE11 family. Chromosomal Location of Human Ortholog: 5q11.2. Cellular Component: cytosol. Molecular Function: protein binding; zinc ion binding; MAP kinase kinase kinase activity; JUN kinase kinase activity; protein kinase binding; ATP binding; protein kinase activity. Biological Process: wound healing; activation of MAPKK activity; apoptosis; regulation of cell migration; protein amino acid phosphorylation; activation of JNK activity; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; apoptotic mitochondrial changes; positive regulation of actin filament polymerization; transforming growth factor beta receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; actin cytoskeleton organization and biogenesis; toll-like receptor 4 signaling pathway. Disease: 46,xy Sex Reversal 6

0.1 mg

315 USD