antibody

Polyclonal Anti-CASP1 (p20) Antibody

MBS176241

0.1 mg

280 USD

polyclonal

rabbit

nonconjugated

mouse, rat

Antibody

Polyclonal Anti-CASP1 (p20) Antibody

[Caspase-1(P20)]

[Caspase-1; Caspase-1; caspase-1; p45; CASP-1; IL-1BC; IL-1B converting enzyme; IL-1 beta-converting enzyme; interleukin-1 beta convertase; interleukin 1 beta-converting enzyme; interleukin-1 beta-converting enzyme; caspase 1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45Cleaved into the following 2 chains:Caspase-1 subunit p20; Caspase-1 subunit p10]

[CASP1]

[Casp1; Casp1; ICE; Il1bc; Il1bc; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme]

CASP1_MOUSE

Polyclonal

IgG

Rabbit

Mouse, Rat

402

No cross reactivity with other proteins.

Immunogen affinity purified.

Lyophilized; Contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

Western Blot:. Concentration: 0.1-0.5ug/ml. Tested Species: Ms, Rat. Predicted Species: - . Antigen Retrieval: -. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Western Blot (WB)

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse CASP1 (137-157aa LEKAQKLWKENPSEIYPIMNT), different from the related rat sequence by three amino acids.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Caspase 1 is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, IL18, and IL33 precursor proteins. It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. The Caspase1 gene consists of 10 exons spanning at least 10.6 kb. The Caspase 1 gene is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal human metaphase chromosomes. Caspase 1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages.

266322

P29452.1

P29452

Amyotrophic Lateral Sclerosis (ALS) Pathway (83296); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Androgen Receptor Signaling Pathway (198319); Apoptosis Pathway (198339); Cytokine Signaling In Immune System Pathway (970814); Cytosolic DNA-sensing Pathway (125146); Cytosolic DNA-sensing Pathway (124833); IL-1 Signaling Pathway (198360); Immune System Pathway (971216); Inflammasomes Pathway (1001249)

CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18. Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. Specifically inhibited by the cowpox virus Crma protein. Belongs to the peptidase C14A family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Apoptosis; EC 3.4.22.36; Protease. Cellular Component: neuron projection; protein complex; mitochondrion; cytoplasm; extracellular region; nucleus. Molecular Function: peptidase activity; protein binding; hydrolase activity; endopeptidase activity; cysteine-type peptidase activity. Biological Process: response to drug; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of apoptosis; apoptosis; membrane hyperpolarization; microglial cell activation; interleukin-1 beta production; response to lipopolysaccharide; mitochondrial depolarization; positive regulation of interleukin-1 beta secretion; proteolysis; response to organic cyclic substance; memory; response to ATP; positive regulation of interleukin-1 alpha secretion; regulation of apoptosis; response to bacterium; regulation of inflammatory response; response to hypoxia; protein processing; regulation of autophagy; positive regulation of circadian sleep/wake cycle, non-REM sleep; myoblast fusion; positive regulation of cytokine secretion

0.1 mg

280 USD