antibody

Anti-Smad4 antibody

MBS176210

0.1 mg

245 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Anti-Smad4 antibody

Smad4

Mothers against decapentaplegic homolog 4; SMAD family member 4; (Small)Mothers Against Decapentaplegic antibody; Deleted in Pancreatic Carcinoma antibody; Deleted in pancreatic carcinoma locus 4 antibody; Deletion target in pancreatic carcinoma 4 antibody; DPC 4 antibody; DPC4 antibody; hSMAD4 antibody; JIP antibody; MAD homolog 4 antibody; MAD mothers against decapentaplegic Drosophila homolog 4 antibody; MAD mothers against decapentaplegic homolog 4 antibody; MADH 4 antibody; MADH4 antibody; Med antibody; Medea antibody; Mothers against decapentaplegic homolog 4 antibody; Mothers against DPP homolog 4 antibody; OTTHUMP00000163548 antibody; SMAD 4 antibody; SMAD family member 4 antibody; SMAD mothers against DPP homolog 4 antibody; Smad4 antibody; SMAD4_HUMAN antibody

Mothers against decapentaplegic homolog 4; Mothers against decapentaplegic homolog 4; mothers against decapentaplegic homolog 4; MAD homolog 4; SMAD, mothers against DPP homolog 4; deleted in pancreatic carcinoma locus 4; deletion target in pancreatic carcinoma 4; mothers against decapentaplegic, Drosophila, homolog of, 4; SMAD family member 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4; SMAD 4; Smad4; hSMAD4

SMAD4

SMAD4; SMAD4; JIP; DPC4; MADH4; MYHRS; DPC4; MADH4; MAD homolog 4; Mothers against DPP homolog 4; SMAD 4; Smad4; hSMAD4

SMAD4_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

552

No cross reactivity with other proteins

Immunogen affinity purified.

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot:. Concentration: 0.1-0.5ug/ml. Tested Species: Hu, Rat. Predicted Species: Ms. Immunohistochemistry (Paraffin-embedded section):. Concentration: 0.5-1ug/ml. Tested Species: Hu, Rat. Predicted Species: Ms. Antigen Retrieval: By Heat. WB: The detection limit for SMAD4 is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sectins in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SMAD4 (97-113aa RLWRWPDLHKNELKHVK), identical to the related rat and mouse sequences.

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 . Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Relevant detection systems: Antibody can be supported by chemiluminescence kit:. MBS176460 in WB. MBS176451 in IHC-(P)

Description: Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 4(SMAD4) detection. Tested with WB, IHC-P in Human, Mouse, Rat. Background: SMAD4(Mothers Against Decapentaplegic Drosophila Homolog of 4), also known as MADH4 or DPC4, is a protein that in humans is encoded by the SMAD4 gene. It belongs to the Darfwin family of proteins that modulate members of the TGFbeta protein superfamily. Hahn et al.(1996) identified the SMAD4 gene on chromosome 18q21.1. Howe et al.(1998) identified the SMAD4 gene within a region on 18q21.1 defined by linkage analysis in a kindred with juvenile polyposis syndrome. To test directly the hypothesis that the SMAD4 gene is a tumor suppressor that is critical for transmitting signals from transforming growth factor-beta and related ligands. SMAD4 plays a pivotal role in signal transduction of the transforming growth factor beta superfamily cytokines by mediating transcriptional activation of target genes.

13959561

Q13485.1

Q13485

60,439 Da

ALK1 Signaling Events Pathway (137968); ALK2 Signaling Events Pathway (137983); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Androgen Receptor Signaling Pathway (198806); BMP Signalling Pathway (545370); BMP Receptor Signaling Pathway (137948); BMP Signalling And Regulation Pathway (198910); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054)

This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Oct 2009]

SMAD4: transcription factor that mediates signal transduction by the transforming growth factor superfamily. The common smad (co-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. Promotes binding of the Smad2/Smad4/Fast-1 complex to DNA and provides an activation function required for Smad1 or Smad2 to stimulate transcription. Protein type: Nuclear receptor co-regulator; DNA-binding; Transcription, coactivator/corepressor. Chromosomal Location of Human Ortholog: 18q21.1. Cellular Component: nucleoplasm; centrosome; transcription factor complex; nuclear chromatin; cytoplasm; cytosol; nucleus. Molecular Function: collagen binding; identical protein binding; protein binding; transforming growth factor beta receptor, common-partner cytoplasmic mediator activity; protein homodimerization activity; DNA binding; sequence-specific DNA binding; metal ion binding; chromatin binding; transcription factor activity. Biological Process: developmental growth; axon guidance; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; sebaceous gland development; negative regulation of transcription from RNA polymerase II promoter; palate development; BMP signaling pathway; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; transforming growth factor beta receptor signaling pathway; mesoderm development; neural crest cell differentiation; positive regulation of BMP signaling pathway; transcription initiation from RNA polymerase II promoter; regulation of transforming growth factor-beta2 production; regulation of binding; transcription, DNA-dependent; in utero embryonic development; neuron fate commitment; positive regulation of transforming growth factor beta receptor signaling pathway; gastrulation with mouth forming second; somite rostral/caudal axis specification; SMAD protein complex assembly; formation of anatomical boundary; endothelial cell activation; cell proliferation; ureteric bud branching; response to hypoxia; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; regulation of hair follicle development; negative regulation of protein catabolic process; negative regulation of transcription, DNA-dependent; endoderm development. Disease: Pancreatic Cancer; Juvenile Polyposis Syndrome; Myhre Syndrome; Juvenile Polyposis/hereditary Hemorrhagic Telangiectasia Syndrome

0.1 mg

245 USD