Anti-NADPH oxidase 4 antibody | MyBioSource MBS176126 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-NADPH oxidase 4 antibody

catalog :

MBS176126

quantity :

0.1 mg

price :

315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Figure 1. Western blot analysis of NADPH oxidase 4/NOX4 using anti-NADPH oxidase 4/NOX4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates,. Lane 2: human SW620 whole cell lysates,. Lane 3: human HK-2 whole cell lysates,. Lane 4: human HL-60 whole cell lysates,. Lane 5: human 293T whole cell lysates,. Lane 6: human SW579 whole cell lysates,. Lane 7: human SK-OV-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NADPH oxidase 4/NOX4 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NADPH OXIDASE 4/NOX4 at approximately 67KD. The expected band size for NADPH OXIDASE 4/NOX4 is at 67KD.

image 2 :

Figure 2. IHC analysis of NADPH OXIDASE 4/NOX4 using anti-NADPH OXIDASE 4/NOX4 antibody (PA1929). NADPH OXIDASE 4/NOX4 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NADPH OXIDASE 4/NOX4 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

image 3 :

Figure 3. Flow Cytometry analysis of U20S cells using anti-NADPH oxidase 4/NOX4 antibody. Overlay histogram showing U20S cells stained (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- NADPH oxidase 4/NOX4 Antibody (1ug/1x10 6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

product information

catalog number :

MBS176126

products type :

Antibody

products full name :

Anti-NADPH oxidase 4 antibody

products short name :

[NADPH oxidase 4]

products name syn :

[NADPH oxidase 4; NADPH oxidase 4; Kidney oxidase-1 antibody; Kidney superoxide-producing NADPH oxidase antibody; KOX 1 antibody; KOX antibody; Kox-1 antibody; NADPH antibody; NADPH oxidase 4 antibody; Nox4 antibody; NOX4_HUMAN antibody; Renal NAD(P)H-oxidase antibody; RENOX antibody]

other names :

[NADPH oxidase 4; NADPH oxidase 4; NADPH oxidase 4; KOX-1; kidney oxidase-1; renal NAD(P)H-oxidase; superoxide-generating NADPH oxidase 4; kidney superoxide-producing NADPH oxidase; NADPH oxidase 4; Kidney oxidase-1; KOX-1; Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase; Superoxide-generating NADPH oxidase 4]

products gene name :

[NOX4]

other gene names :

[Nox4; Nox4; AI648021; Renox; KOX-1]

uniprot entry name :

NOX4_MOUSE

clonality :

Polyclonal

isotype :

Rabbit IgG

host :

Rabbit

reactivity :

Human, Rat; Predicted to work with: Mouse

sequence length :

578

specificity :

No cross reactivity with other proteins

purity :

Immunogen affinity purified.

form :

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

storage stability :

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

app notes :

Western Blot:. Concentration:0.1-0.5ug/ml. Tested Species: Hu, Rat. Predicted Species: Ms. Immunohistochemistry (Paraffin-embedded Section): . Concentration:0.5-1ug/ml . Tested Species: Rat . Predicted Species: Hu,Ms . Antigen Retrieval: By Heat. Tested Species: In house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Flow Cytometry (FC/FACS)

image4 heading :

Western Blot (WB)

image4 description :

Figure 4. Western blot analysis of NADPH oxidase 4/NOX4 using anti-NADPH oxidase 4/NOX4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NADPH oxidase 4/NOX4 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. Specific bands were detected for NADPH oxidase 4/NOX4 at approximately 67-80KD. The expected band size for NADPH oxidase 4/NOX4 are at 67-80KD.

other info1 :

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse NOX4(561-578aa NRNNSYGTKFEYNKES), identical to the related rat sequence and different from the related human sequence by two amino acids.

other info2 :

Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

products description :

NADPH oxidase 4 is an enzyme that in humans is encoded by the NOX4 gene, and is a member of the NOX family of NADPH oxidases. This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants

ncbi gi num :

81881891

ncbi acc num :

Q9JHI8.1

uniprot acc num :

Q9JHI8

ncbi pathways :

Spinal Cord Injury Pathway (739004)

uniprot summary :

NOX4: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Oxidoreductase; Nucleolus; EC 1.6.3.-; Membrane protein, multi-pass; Membrane protein, integral; Cell cycle regulation. Cellular Component: focal adhesion; mitochondrion; membrane; endoplasmic reticulum; perinuclear region of cytoplasm; apical plasma membrane; integral to membrane; stress fiber; plasma membrane; cell junction; NADPH oxidase complex. Molecular Function: NAD(P)H oxidase activity; superoxide-generating NADPH oxidase activity; oxidoreductase activity, acting on NADH or NADPH, with oxygen as acceptor; oxidoreductase activity. Biological Process: positive regulation of apoptosis; cell morphogenesis; superoxide metabolic process; cell aging; homocysteine metabolic process; positive regulation of smooth muscle cell migration; cardiac muscle cell differentiation; positive regulation of protein kinase B signaling cascade; positive regulation of MAP kinase activity; negative regulation of cell proliferation; positive regulation of stress fiber formation; superoxide release; bone resorption

size1 :

0.1 mg

price1 :

315 USD

more info or order :

MyBioSource product webpage