Anti-Phospholipase A2 antibody | MyBioSource MBS176121 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Phospholipase A2 antibody

catalog :

MBS176121

quantity :

0.1 mg

price :

245 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS176121

products type :

Antibody

products full name :

Anti-Phospholipase A2 antibody

products short name :

Phospholipase A2

products name syn :

Cytosolic phospholipase A2; phospholipase A2, group IVA(cytosolic, calcium-dependent); Calcium dependent phospholipid binding protein antibody; CPLA 2 antibody; cPLA2 alpha antibody; cPLA2 antibody; Cytosolic phospholipase A2 antibody; Cytosolic phospholipase A2 group IVA antibody; Lysophospholipase antibody; MGC126350 antibody; PA24A_HUMAN antibody; Phosphatidylcholine 2 acylhydrolase antibody; Phosphatidylcholine 2-acylhydrolase antibody; Phospholipase A2 group 4 A antibody; Phospholipase A2 group IVA(cytosolic calcium dependent) antibody; Phospholipase A2 group IVA antibody; PhospholipaseA2 antibody; PLA2G4 antibody; pla2g4a antibody

other names :

Cytosolic phospholipase A2; Cytosolic phospholipase A2; cytosolic phospholipase A2; cPLA2; lysophospholipase; phospholipase A2 group IVA; phosphatidylcholine 2-acylhydrolase; calcium-dependent phospholipid-binding protein; phospholipase A2, group IVA (cytosolic, calcium-dependent); Phospholipase A2 group IVAIncluding the following 2 domains:Phospholipase A2 (EC:3.1.1.4)Alternative name(s):Phosphatidylcholine 2-acylhydrolase

products gene name :

PLA2G4A

other gene names :

PLA2G4A; PLA2G4A; PLA2G4; cPLA2-alpha; CPLA2; PLA2G4; cPLA2

uniprot entry name :

PA24A_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

749

purity :

Immunogen affinity purified.

form :

Lyophilized

storage stability :

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB)

other info1 :

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Phospholipase A2 (686-700aa PNQAFKRLHDLMHFN), different from the related rat and mouse sequences by one amino acid.

other info2 :

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

products description :

Description: Rabbit IgG polyclonal antibody for Cytosolic phospholipase A2(PLA2G4A) detection. Tested with WB in Human, Mouse, Rat. Background: PLA2G4A(Phospholipase A2, Group IVA), is an enzyme that in humans is encoded by the PLA2G4A gene. Tay et al.(1995) mapped the PLA2G4A gene to rat chromosome 13 by PCR-based intercross genotyping and to human 1q25 by fluorescence in situ hybridization. By site-directed mutagenesis and biochemical analysis of the recombinant protein, Sharp et al.(1994) determined that ser228 participates in the catalytic mechanism of cPLA2 and that both the phospholipase A2 and the lysophospholipase activities are catalyzed by the same active site residue(s). PLA2G4A, the cytosolic phospholipase A2, appears to subserve transmembrane signaling responses to extracellular ligands(Skorecki, 1995).

ncbi gi num :

317373312

ncbi acc num :

P47712.2

uniprot acc num :

P47712

ncbi mol weight :

85,239 Da

ncbi pathways :

ADP Signalling Through P2Y Purinoceptor 1 Pathway (161061); AGE/RAGE Pathway (698754); Acyl Chain Remodeling Of CL Pathway (645330); Acyl Chain Remodelling Of PC Pathway (645321); Acyl Chain Remodelling Of PE Pathway (645317); Acyl Chain Remodelling Of PG Pathway (645328); Acyl Chain Remodelling Of PI Pathway (645326); Acyl Chain Remodelling Of PS Pathway (645324); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553)

ncbi summary :

This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. [provided by RefSeq, Jul 2008]

uniprot summary :

cPLA2: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Arachidonic acid is a precursor for the eicosanoids that are involved in hemodynamic regulation, inflammatory responses, and other cellular processes. Promotes cerebellar long-term depression and motor learning. Translocates to the Golgi and endoplasmic reticulum in a calcium-dependent fashion. Translocation and activation of at the ER facilitates the process of ER stress. Regulates the biogenesis of lipid droplets, a process dependent on JNK and ceramide kinase. Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+. The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Inhibited when in trimolecular complex with ANXA2 and S100A10. Phosphorylation of S727 relieves this inhibitory interaction, thus activating PLA2G4A. Protein type: Lipid Metabolism - linoleic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; EC 3.1.1.4; Lipid Metabolism - alpha-linolenic acid; Phospholipase; EC 3.1.1.5; Lipid Metabolism - arachidonic acid. Chromosomal Location of Human Ortholog: 1q25. Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; mitochondrial inner membrane; cytoplasmic membrane-bound vesicle; cytoplasm; cytosol. Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; calcium-dependent phospholipid binding; calcium ion binding; lysophospholipase activity. Biological Process: icosanoid biosynthetic process; platelet activating factor biosynthetic process; platelet activation; phospholipid catabolic process; phospholipid metabolic process; glycerophospholipid biosynthetic process; icosanoid metabolic process; arachidonic acid metabolic process; phosphatidic acid biosynthetic process; arachidonic acid secretion; blood coagulation; regulation of cell proliferation

size1 :

0.1 mg

price1 :

245 USD

more info or order :

MyBioSource product webpage