antibody

Anti-Cytochrome P450 1A2 antibody

MBS176066

0.1 mg

245 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Anti-Cytochrome P450 1A2 antibody

Cytochrome P450 1A2

Cytochrome P450 1A2; cytochrome P450, family 1, subfamily A, polypeptide 2; Aryl hydrocarbon hydroxylase antibody; CP 12 antibody; CP12 antibody; CP1A2_HUMAN antibody; CYP1A2 antibody; CYPIA2 antibody; Cytochrome P(3)450 antibody; Cytochrome P450 1A2 antibody; Cytochrome P450 4 antibody; Cytochrome P450 family 1 polypeptide 2 antibody; Cytochrome P450 family 1 subfamily A polypeptide 2 antibody; Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2 antibody; Cytochrome P450-P3 antibody; Cytochrome P4501A2 antibody; Dioxin inducable P3 450 antibody; Flavoprotein linked monooxygenase antibody; Microsomal monooxygenase antibody; P(3)450 antibody; P3 450 antibody; P450 4 antibody; P450 form 4 antibody; P450 P3 antibody; P450(PA) antibody; Xenobiotic monooxygenase antibody

Cytochrome P450 1A2; Cytochrome P450 1A2; cytochrome P450 1A2; CYPIA2; P450 form 4; cytochrome P450 4; cytochrome P(3)450; cytochrome P450-P3; dioxin-inducible P3-450; microsomal monooxygenase; xenobiotic monooxygenase; aryl hydrocarbon hydroxylase; flavoprotein-linked monooxygenase; cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2; cytochrome P450, family 1, subfamily A, polypeptide 2; CYPIA2; Cytochrome P(3)450; Cytochrome P450 4; Cytochrome P450-P3

CYP1A2

CYP1A2; CYP1A2; CP12; P3-450; P450(PA)

CP1A2_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

515

Immunogen affinity purified.

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot (WB): . Concentration: 0.1-0.5ug/ml; Tested Species: Hu, Rat; Predicted Species: Ms. Immunohistochemistry (Paraffin-embeded Section). Concentration: 0.5-1ug/ml; Tested Species: Rat; Predicted Species: Hu, Ms; Antigen Retrieval: By Heat. WB: The detection limit for CYP1A2 is approximately 1ng/lane under reducing conditions. . Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Cytochrome P450 1A2 (265-282aa QKTVQEHYQDFDKNSVRD), different from the related rat and mouse sequences by three amino acids.

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Description: Rabbit IgG polyclonal antibody for Cytochrome P450 1A2(CYP1A2) detection. Tested with WB, IHC-P in Human, Mouse, Rat. Background: CYP1A2(Cytochrome P450, Subfamily I, Polypeptide 2) is a member of the cytochrome P450 mixed-function oxidase system and is involved in the metabolism of xenobiotics in the body. CYP1A2 is a member of the cytochrome P450 superfamily of enzymes. In humans, the CYP1A2 enzyme is encoded by the CYP1A2 gene. CYP1A2 localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons(PAHs), some of which are found in cigarette smoke. The CYP1A2 gene encodes a P450 enzyme involved in O-deethylation of phenacetin. Ikeya et al.(1989) found that the human CYP1A2 gene spans almost 7.8 kb and contains 7 exons. The CYP1A2 gene is mapped on 15q24.1. CYP1A2 accounts for nearly 15% of the cytochrome P450 in the human liver. CYP1A2 displays higher activity in men than in women, and is inhibited by oral contraceptives. Inducers of CYP1A2 include cruciferous vegetables. Cigarette smoking has also been shown to increaseCYP1A2 activity. Expression of CYP1A2 appears to be induced by various dietary constituents.

117144

P05177.3

P05177

58,407 Da

Aflatoxin B1 Metabolism Pathway (198808); Aflatoxin Activation And Detoxification Pathway (1016173); AhR Pathway (755436); Arachidonic Acid Metabolism Pathway (685553); Aromatic Amines Can Be N-hydroxylated Or N-dealkylated By CYP1A2 Pathway (105704); Arylamine Metabolism Pathway (198830); Biological Oxidations Pathway (105698); Caffeine Metabolism Pathway (82945); Caffeine Metabolism Pathway (308); Chemical Carcinogenesis Pathway (673221)

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region. [provided by RefSeq, Jul 2008]

CYP1A2: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O- deethylation of phenacetin. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Lipid Metabolism - linoleic acid; Amino Acid Metabolism - tryptophan; Oxidoreductase; EC 1.14.14.1; Secondary Metabolites Metabolism - caffeine. Chromosomal Location of Human Ortholog: 15q24.1. Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle. Molecular Function: enzyme binding; electron carrier activity; iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxidoreductase activity; monooxygenase activity; demethylase activity. Biological Process: methylation; dibenzo-p-dioxin metabolic process; cellular respiration; monoterpenoid metabolic process; epoxygenase P450 pathway; response to lipopolysaccharide; toxin biosynthetic process; monocarboxylic acid metabolic process; response to estradiol stimulus; post-embryonic development; drug catabolic process; regulation of gene expression; xenobiotic metabolic process; porphyrin metabolic process; exogenous drug catabolic process; arachidonic acid metabolic process; alkaloid metabolic process; heterocycle metabolic process; drug metabolic process; hydrogen peroxide biosynthetic process; steroid catabolic process; lung development

0.1 mg

245 USD