Anti-Bone Sialoprotein antibody | MyBioSource MBS176061 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Bone Sialoprotein antibody

catalog :

MBS176061

quantity :

0.1 mg

price :

315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-Bone Sialoprotein antibody, MBS176061, IHC(P). IHC(P): Rat Liver Tissue

image 2 :

Anti-Bone Sialoprotein antibody, MBS176061, Western blotting. Lane 1: Rat Liver Tissue Lysate. Lane 2: Rat Brain Tissue Lysate. Lane 3: Rat Kidney Tissue Lysate.

product information

catalog number :

MBS176061

products type :

Antibody

products full name :

Anti-Bone Sialoprotein antibody

products short name :

[Bone Sialoprotein]

products name syn :

[Bone sialoprotein 2; integrin-binding sialoprotein; BNSP antibody; Bone sialoprotein 2 antibody; Bone sialoprotein II antibody; BSP antibody; BSP II antibody; BSPII antibody; Cell binding sialoprotein antibody; Cell-binding sialoprotein antibody; IBSP antibody; Integrin binding sialoprotein antibody; Integrin-binding sialoprotein antibody; SIAL_HUMAN antibody; SPII antibody]

other names :

[Bone sialoprotein 2; Bone sialoprotein 2; bone sialoprotein 2; bone sialoprotein II; cell-binding sialoprotein; integrin-binding sialoprotein; integrin binding sialoprotein; Bone sialoprotein II; BSP II; Cell-binding sialoprotein; Integrin-binding sialoprotein]

products gene name :

[IBSP]

other gene names :

[Ibsp; Ibsp; BSP; Bsp2; BSPII; BSP II; BSP II]

uniprot entry name :

SIAL_MOUSE

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse, Rat

sequence length :

324

specificity :

No cross reactivity with other proteins.

purity :

Immunogen affinity purified.

form :

Lyophilized

storage stability :

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

app notes :

Western Blot:. Concentration: 0.1-0.5ug/ml. Tested Species: Rat. Predicted Species: Ms. Immunohistochemistry (Paraffin-embedded Section): . Concentration: 0.5-1ug/ml. Tested Species: Ms, Rat. Antigen Retrieval: By Heat. WB: The detection limit for IBSP is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

other info1 :

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bone Sialoprotein (305-324aa SYYKGHGYEGYEGQNYYYHQ), different from the related rat sequence by one amino acid.

other info2 :

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

products description :

Description: Rabbit IgG polyclonal antibody for Bone sialoprotein 2(IBSP) detection. Tested with WB, IHC-P in Mouse, Rat. Background: IBSP(integrin-binding sialoprotein) is also known as BSP. The protein encoded by this gene is a major structural protein of the bone matrix. Bone sialoprotein is an acidic glycoprotein of approximately 70 kD that undergoes extensive posttranslational modifications. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. The BSP gene is mapped to 4q22.1.

ncbi gi num :

341942035

ncbi acc num :

Q61711.2

uniprot acc num :

Q61711

ncbi pathways :

ECM Proteoglycans Pathway (1001027); ECM-receptor Interaction Pathway (83265); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (1001015); Focal Adhesion Pathway (198353); Focal Adhesion Pathway (83264); Focal Adhesion Pathway (478); Integrin Cell Surface Interactions Pathway (1001031); PI3K-Akt Signaling Pathway (692242); PI3K-Akt Signaling Pathway (692979)

uniprot summary :

Sialoprotein-2: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment. Protein type: Secreted; Cell adhesion; Secreted, signal peptide; Motility/polarity/chemotaxis. Cellular Component: extracellular space; membrane; extracellular region. Biological Process: osteoblast differentiation; ossification; extracellular matrix organization and biogenesis; biomineral formation; cell adhesion

size1 :

0.1 mg

price1 :

315 USD

more info or order :

MyBioSource product webpage