antibody

Anti-Glutaredoxin 2 antibody

MBS176047

0.1 mg

315 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Anti-Glutaredoxin 2 antibody

[Glutaredoxin 2]

[Glutaredoxin-2, mitochondrial; glutaredoxin 2; bA101E13.1(GRX2 glutaredoxin(thioltransferase) 2) antibody; bA101E13.1 antibody; CGI133 antibody; Glrx2 antibody; GLRX2_HUMAN antibody; Glutaredoxin-2 antibody; Glutaredoxin-2, mitochondrial antibody; GRX2 antibody; mitochondrial antibody]

[Glutaredoxin-2, mitochondrial; Glutaredoxin-2, mitochondrial; glutaredoxin 2; bA101E13.1 (GRX2 glutaredoxin (thioltransferase) 2); glutaredoxin 2]

[GLRX2]

[GLRX2; GLRX2; GRX2; CGI-133; GRX2]

GLRX2_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

164

Immunogen affinity purified.

Lyophilized

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western blot . Concentration: 0.1-0.5 ug/mL. Tested Species: Hu, Rat. Predicted Species: Ms. Immunohistochemistry (Paraffin-embedded Section) . Concentration: 0.5-1 ug/mL. Tested Species: Hu, Rat. Predicted Species: Ms. Antigen Retrieval: By Heat. Tested Species: In- house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0 for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Western Blot (WB)

Immunohistochemistry (IHC)

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Glutaredoxin 2 (103-119aa EYGNQFQDALYKMTGER), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.

Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Description: Rabbit IgG polyclonal antibody for Glutaredoxin-2, mitochondrial(GLRX2) detection. Tested with WB, IHC-P in Human, Mouse, Rat. Background: GLRX2(Glutaredoxin-2) also known as Glutaredoxin-2, mitochondrial or GRX2, is a protein that in humans is encoded by the GLRX2 gene. Glutaredoxins(e.g., GLRX) are a family of glutathione-dependent hydrogen donors that participate in a variety of cellular redox reactions. By sequence analysis, Lundberg et al.(2001) and Gladyshev et al.(2001) identified the GLRX2 gene within a clone mapping to chromosome 1q31.2-q31.3. Lundberg et al.(2001) determined that the GLRX2 gene contains 5 exons spanning about 9.6 kb. The GLRX2B transcript uses a first exon(exon 1B) located upstream from the first exon used by the GLRX2A transcript(exon 1A), suggesting that alternative splicing generates the isoforms. Lundberg et al.(2001)assayed reductase activity in recombinant proteins of both GLRX2 isoforms. They found that both have GSH-dependent dehydroascorbate reductase activity and 2-hydroxyethyl disulfide reductase activity.

73919686

Q9NS18.1

Q9NS18

18,726 Da

The protein encoded by this gene is a member of the glutaredoxin family of proteins, which maintain cellular thiol homeostasis. These proteins are thiol-disulfide oxidoreductases that use a glutathione-binding site and one or two active cysteines in their active site. This gene undergoes alternative splicing to produce multiple isoforms, one of which is ubiquitously expressed and localizes to mitochondria, where it functions in mitochondrial redox homeostasis and is important for the protection against and recovery from oxidative stress. Other isoforms, which have more restrictive expression patterns, show cytosolic and nuclear localization, and are thought to function in cellular differentiation and transformation, possibly with a role in tumor progression. [provided by RefSeq, Aug 2011]

GLRX2: Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release. Belongs to the glutaredoxin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Oxidoreductase; Mitochondrial. Chromosomal Location of Human Ortholog: 1q31.2. Cellular Component: nucleoplasm; mitochondrion; intracellular membrane-bound organelle; nucleus. Molecular Function: 2 iron, 2 sulfur cluster binding; electron carrier activity; metal ion binding; protein disulfide oxidoreductase activity; protein disulfide isomerase activity; arsenate reductase (glutaredoxin) activity; glutathione disulfide oxidoreductase activity. Biological Process: response to organic substance; response to temperature stimulus; DNA protection; glutathione metabolic process; response to hydrogen peroxide; regulation of transcription, DNA-dependent; protein folding; apoptosis; cell redox homeostasis; regulation of signal transduction; response to redox state; cell differentiation

0.1 mg

315 USD