ELISA/assay

Human BDNF PicoKine ELISA Kit

MBS176010

96 Wells

325 USD

ELISA Kit

Human BDNF PicoKine ELISA Kit

BDNF

Brain-derived neurotrophic factor; brain-derived neurotrophic factor; Abrineurin; ANON2; Brain derived neurotrophic factor; BULN2; MGC34632; Neurotrophin

Brain-derived neurotrophic factor; Brain-derived neurotrophic factor; brain-derived neurotrophic factor; abrineurin; neurotrophin; brain-derived neurotrophic factor; Abrineurin

BDNF

BDNF; BDNF; ANON2; BULN2; BDNF

BDNF_HUMAN

Human

247

Natural and recombinant human BDNF

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate). Assay Type: Sandwich. Detection Range: 31.2pg/ml-2000pg/ml. Sensitivity: < 2pg/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Principle of the assay: human BDNF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BDNF has been precoated onto 96-well plates. Standards(sf21, H129-R247) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BDNF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BDNF amount of sample captured in plate. Background: Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system.1 BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediatedLTP is induced postsynaptically.2 BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervoussystem.3 BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in foodintake through central mediators in both the basal and fasted state.4 It plays a role in treating breathing disorderssuch as respiratory insufficiency after spinal injury.5 The mature form of BDNF is identical in all mammalsexamined, and the gene encoding human BDNF to chromosome 11, band p13. 6

114900

P23560.1

P23560

31,116 Da

Alcoholism Pathway (585563); Alcoholism Pathway (587116); BDNF Signaling Pathway (712093); Cocaine Addiction Pathway (546258); Cocaine Addiction Pathway (546273); FSH Signaling Pathway (672455); Huntington's Disease Pathway (83100); Huntington's Disease Pathway (512); Integrated Pancreatic Cancer Pathway (711360); MAPK Signaling Pathway (198779)

The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jan 2009]

BDNF: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. Belongs to the NGF-beta family. 5 isoforms of the human protein are produced by alternative promoter. Protein type: Secreted; Cell development/differentiation; Secreted, signal peptide; Cytokine. Chromosomal Location of Human Ortholog: 11p13. Cellular Component: extracellular space; mitochondrial crista; synaptic vesicle; perinuclear region of cytoplasm; cytoplasmic membrane-bound vesicle; cytoplasm; dendrite; extracellular region; terminal button; perikaryon. Molecular Function: growth factor activity; neurotrophin TRKB receptor binding. Biological Process: circadian rhythm; axon guidance; mechanoreceptor differentiation; behavioral fear response; mitochondrial electron transport, NADH to ubiquinone; regulation of neuron differentiation; axon extension; response to hormone stimulus; positive regulation of long-term neuronal synaptic plasticity; response to vitamin A; negative regulation of neuroblast proliferation; ureteric bud development; dendrite development; regulation of retinal cell programmed cell death; feeding behavior; negative regulation of neuron apoptosis; negative regulation of synaptic transmission, GABAergic; response to electrical stimulus; inner ear development; response to food; nervous system development; chronic inflammatory response; response to light intensity; neuron recognition; learning; regulation of axon extension; regulation of short-term neuronal synaptic plasticity; negative regulation of striated muscle development; positive regulation of peptidyl-serine phosphorylation; positive regulation of synaptogenesis; response to hyperoxia; axon target recognition; glutamate secretion; response to hypoxia; response to fluoxetine; nerve development; response to activity; positive regulation of neuron differentiation; regulation of excitatory postsynaptic membrane potential; neurite morphogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; gamma-aminobutyric acid signaling pathway. Disease: Bulimia Nervosa, Susceptibility To, 1; Obsessive-compulsive Disorder; Bulimia Nervosa, Susceptibility To, 2; Central Hypoventilation Syndrome, Congenital

96 Wells

325 USD