ELISA/assay

Rat IL-10 PicoKine ELISA Kit

MBS175998

96 Wells

410 USD

ELISA Kit

Rat IL-10 PicoKine ELISA Kit

IL-10

Interleukin-10; interleukin 10; CSIF; Cytokine synthesis inhibitory factor; GVHDS; IL 10; IL-10; IL10; IL10_HUMAN; IL10A; Interleukin 10; Interleukin-10; Interleukin10; MGC126450; MGC126451; T cell growth inhibitory factor; TGIF

Interleukin-10; Interleukin-10; interleukin-10; CSIF; IL-10; cytokine synthesis inhibitory factor; interleukin 10; Cytokine synthesis inhibitory factor; CSIF

IL10

Il10; Il10; IL10X; Il-10; IL-10; CSIF

IL10_RAT

Rat

178

Natural and recombinant rat IL-10

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates, serum and plasma (EDTA). Assay Type: Sandwich. Detection Range: 31.2pg/ml-2000pg/ml. Sensitivity: < 4pg/ml

Principle of the assay: rat IL-10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-10 has been precoated onto 96-well plates. Standards (E.coli,S19-N178) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-10 amount of sample captured in plate. Background: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production.1 IL-10 is animportant cytokine with anti-inflammatory, anti-immune, and antifibrotic functions.2 It is also an important regulatorycytokine whose involvement extends into diverse areas of the human immune system.3 IL-10 is a recentlydescribed natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8.4 Interleukin-10 gene polymorphisms and interleukin-10 production capability maycontribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10locus contributes to the heritability of psoriasis susceptibility.5 With regard to sudden infant death, IL-10 is ofspecial interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease.6 The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to humanchromosome 1.7.

266338

P29456.1

P29456

20,426 Da

African Trypanosomiasis Pathway (194377); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83511); Allograft Rejection Pathway (535); Amoebiasis Pathway (167311); Amoebiasis Pathway (167191); Asthma Pathway (83508); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83509); Autoimmune Thyroid Disease Pathway (533)

factor involved in the inhibition of cytokine synthesis [RGD, Feb 2006]

96 Wells

410 USD