ELISA/assay

Human IL-10 PicoKine ELISA Kit

MBS175968

96 Wells

305 USD

ELISA Kit

Human IL-10 PicoKine ELISA Kit

IL-10

Interleukin-10; interleukin 10; CSIF; Cytokine synthesis inhibitory factor; GVHDS; IL 10; IL-10; IL10; IL10_HUMAN; IL10A; Interleukin 10; Interleukin-10; Interleukin10; MGC126450; MGC126451; T cell growth inhibitory factor; TGIF

Interleukin-10; Interleukin-10; interleukin-10; T-cell growth inhibitory factor; cytokine synthesis inhibitory factor; interleukin 10; Cytokine synthesis inhibitory factor; CSIF

IL10

IL10; IL10; CSIF; TGIF; GVHDS; IL-10; IL10A; IL-10; CSIF

IL10_HUMAN

Human

178

Natural and recombinant human IL-10

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate). Assay Type: Sandwich. Detection Range: 7.8pg/ml-500pg/ml(cell culture supernates) 3.4pg/ml-250pg/ml(human serum, plasma). Sensitivity: < 0.5pg/ml

Principle of the assay: human IL-10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-10 has been precoated onto 96-well plates. Standards (E.coli,S19-N178) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-10 amount of sample captured in plate. Background: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production.1 IL-10 is an importantcytokine with anti-inflammatory, anti-immune, and antifibrotic functions.2 It is also an important regulatory cytokinewhose involvement extends into diverse areas of the human immune system.3 IL-10 is a recently described naturalendogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8.4 Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to thedevelopment of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes tothe heritability of psoriasis susceptibility.5 With regard to sudden infant death, IL-10 is of special interest. This is animmunoregulatory cytokine that plays an important role in the development of infectious disease.6 The mIL-10 geneis mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.7 The standardproduct used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.

124292

P22301.1

P22301

20,517 Da

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Asthma Pathway (83120); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83121)

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]

IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family. Protein type: Motility/polarity/chemotaxis; Cytokine; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 1q31-q32. Cellular Component: extracellular space. Molecular Function: growth factor activity; cytokine activity; interleukin-10 receptor binding. Biological Process: B cell proliferation; negative regulation of chronic inflammatory response to antigenic stimulus; regulation of isotype switching; positive regulation of transcription, DNA-dependent; leukocyte chemotaxis; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; negative regulation of B cell proliferation; negative regulation of membrane protein ectodomain proteolysis; response to insulin stimulus; negative regulation of interferon-alpha biosynthetic process; negative regulation of T cell proliferation; cytoplasmic sequestering of NF-kappaB; cell-cell signaling; positive regulation of MHC class II biosynthetic process; negative regulation of interferon-gamma production; response to molecule of bacterial origin; negative regulation of interleukin-6 production; hemopoiesis; negative regulation of tumor necrosis factor biosynthetic process; inflammatory response; aging; response to drug; response to inactivity; negative regulation of interleukin-12 production; positive regulation of B cell apoptosis; T-helper 2 type immune response; negative regulation of myeloid dendritic cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of cytokine secretion during immune response; regulation of gene expression; B cell differentiation; defense response to bacterium; negative regulation of MHC class II biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; receptor biosynthetic process; response to activity; regulation of sensory perception of pain; negative regulation of apoptosis; positive regulation of cytokine secretion. Disease: Graft-versus-host Disease, Susceptibility To; Rheumatoid Arthritis; Human Immunodeficiency Virus Type 1, Susceptibility To

96 Wells

305 USD