ELISA/assay

Mouse IL-1 beta PicoKine ELISA Kit

MBS175967

96-Strip-Wells

475 USD

ELISA Kit

Mouse IL-1 beta PicoKine ELISA Kit

[IL-1 beta]

[Interleukin-1 beta; interleukin 1, beta; Catabolin; Hematopoietin 1; IFN beta inducing factor; IL 1; IL 1 beta; IL 1B; IL-1 beta; IL1; IL1 BETA; IL1B; IL1B_HUMAN; IL1F2; Interleukin 1 beta; Interleukin 1 beta precursor; Interleukin-1 beta; LAF; OAF; Osteoclast activating factor; Preinterleukin beta; Pro interleukin 1 beta]

[Interleukin-1 beta; Interleukin-1 beta; interleukin-1 beta; IL-1 beta; interleukin 1 beta]

[IL1B]

[Il1b; Il1b; Il-1b; IL-1beta; IL-1 beta]

IL1B_MOUSE

Mouse

269

Natural and recombinant mouse IL-1?

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Detection Range: 12.5pg/ml-800pg/ml. Sensitivity: < 1pg/ml

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Principle of the Assay: The mouse IL-1? ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-1? has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: V118-S269) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1? is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-1? amount of sample captured in plate. Background: Interleukin-1? (IL-1?) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1?, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1? and, to a lesser extent, IL-1? are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1? and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1?, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

124304

P10749.1

P10749

30,931 Da

African Trypanosomiasis Pathway (194387); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Apoptosis Pathway (83257); Apoptosis Pathway (470); Chagas Disease (American Trypanosomiasis) Pathway (147810); Chagas Disease (American Trypanosomiasis) Pathway (147795)

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response. [provided by RefSeq, Aug 2015]

IL1B: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Monomer. Belongs to the IL-1 family. Protein type: Cytokine. Cellular Component: extracellular space; cell; extracellular region; intracellular; cytosol; vesicle; secretory granule. Molecular Function: protein domain specific binding; interleukin-1 receptor binding; cytokine activity; receptor binding. Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; negative regulation of MAP kinase activity; positive regulation of JNK activity; positive regulation of nitric oxide biosynthetic process; negative regulation of glutamate secretion; activation of MAPK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of interleukin-2 biosynthetic process; germ cell programmed cell death; negative regulation of insulin receptor signaling pathway; positive regulation of glial cell differentiation; response to lipopolysaccharide; positive regulation of lipid catabolic process; positive regulation of NF-kappaB import into nucleus; fever; positive regulation of membrane protein ectodomain proteolysis; response to carbohydrate stimulus; activation of NF-kappaB transcription factor; elevation of cytosolic calcium ion concentration; positive regulation of phagocytosis; positive regulation of T cell proliferation; positive regulation of astrocyte differentiation; neutrophil chemotaxis; positive regulation of heterotypic cell-cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; interleukin-1 beta production; positive regulation of interleukin-6 production; social behavior; negative regulation of neuron differentiation; positive regulation of angiogenesis; positive regulation of cell division; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of lipid metabolic process; leukocyte migration; sequestering of triacylglycerol; positive regulation of interleukin-6 biosynthetic process; positive regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; positive regulation of stress-activated MAPK cascade; negative regulation of neurogenesis; positive regulation of interleukin-8 production; negative regulation of cell proliferation; negative regulation of lipid catabolic process; hyaluronan biosynthetic process; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; regulation of I-kappaB kinase/NF-kappaB cascade; inflammatory response; aging; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; positive regulation of immature T cell proliferation in the thymus; memory; response to ATP; positive regulation of interferon-gamma production; positive regulation of chemokine biosynthetic process; positive regulation of prostaglandin secretion; positive regulation of fever; immune response; positive regulation of protein amino acid phosphorylation; regulation of insulin secretion

96-Strip-Wells

475 USD