ELISA/assay

Human IL-1 alpha PicoKine ELISA Kit

MBS175942

96 Wells

320 USD

ELISA Kit

Human IL-1 alpha PicoKine ELISA Kit

IL-1 alpha

Interleukin-1 alpha; interleukin 1, alpha; Hematopoietin 1; IL 1 alpha; Il 1a; IL1; IL1 ALPHA; IL1A; IL1F1; Ilia; Interleukin 1 alpha; Interleukin 1 alpha precursor; Interleukin1 alpha; OTTHUMP00000203692; OTTMUSP00000016430; Preinterleukin 1; Pro interleukin 1; Pro interleukin 1 alpha; Prointerleukin 1; RP23-160G19.8

Interleukin-1 alpha; Interleukin-1 alpha; interleukin-1 alpha; IL-1 alpha; hematopoietin-1; preinterleukin 1 alpha; pro-interleukin-1-alpha; interleukin 1, alpha; Hematopoietin-1

IL1A

IL1A; IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA; IL1F1; IL-1 alpha

IL1A_HUMAN

Human

271

Natural and recombinant human IL-1alpha

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate). Assay Type: Sandwich. Detection Range: 4.7pg/ml-300pg/ml. Sensitivity: < 0.5pg/ml

Principle of the assay: human IL-1alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1alpha has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-1alpha amount of sample captured in plate. Background: Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair.1 IL-1 alpha hasbeen implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases.2 Recently, it has beenshown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene isassigned to chromosome 2.3 Genetic polymorphisms at interleukin (IL)-1alpha and IL-1beta have been recentlysuggested to be associated with severity of adult periodontitis.4 The murine IL-1 alpha and IL-1 beta genes encodestructurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis.5 The standard product used in this kit is recombinant human IL-1alpha with the molecular massof 18kDa.

124297

P01583.1

P01583

30,607 Da

Apoptosis Pathway (83060); Apoptosis Pathway (470); Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); FAS Pathway And Stress Induction Of HSP Regulation (198894); Graft-versus-host Disease Pathway (83124)

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]

IL1A: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Belongs to the IL-1 family. Protein type: Motility/polarity/chemotaxis; Cytokine. Chromosomal Location of Human Ortholog: 2q14. Cellular Component: extracellular space; extracellular region; cytosol. Molecular Function: protein binding; copper ion binding; interleukin-1 receptor binding; cytokine activity. Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; apoptosis; positive regulation of interleukin-2 biosynthetic process; cytokine and chemokine mediated signaling pathway; germ cell programmed cell death; positive regulation of JNK cascade; fever; negative regulation of cell proliferation; cell proliferation; connective tissue replacement during inflammatory response; positive regulation of angiogenesis; response to copper ion; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response; positive regulation of cytokine secretion

96 Wells

320 USD