ELISA/assay

Rat ICAM-1 PicoKine ELISA Kit

MBS175919

96 Wells

455 USD

ELISA Kit

Rat ICAM-1 PicoKine ELISA Kit

ICAM-1

Intercellular adhesion molecule 1; intercellular adhesion molecule 1; BB2; CD 54; CD54; CD54 antigen; Cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM 1; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA2; MGC6195; MyD10; P3.58; Surface antigen of activated B cells

Intercellular adhesion molecule 1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1; ICAM-1; cell surface glycoprotein P3.58; major group rhinovirus receptor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; CD_antigen: CD54

ICAM1

ICAM1; ICAM1; BB2; CD54; P3.58; ICAM-1

ICAM1_HUMAN

Rat

532

Natural and recombinant rat ICAM-1

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates, serum and plasma (heparin, EDTA). Assay Type: Sandwich. Detection Range: 31.2pg/ml-2000pg/ml. Sensitivity: < 2pg/ml

Principle of the assay: rat ICAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ICAM-1 has been precoated onto 96-well plates. Standards(NSO,Q28 - T493) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ICAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat ICAM-1 amount of sample captured in plate. Background: Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin.1 Thenormal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1).2 ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cellsand its expression is regulated by inflammatory cytokines.3 ICAM-1 has a tissue distribution similar to that of themajor histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1 Thestandard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds.

68067956

P05362.2

P05362

57,825 Da

Adaptive Immune System Pathway (366160); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cytokine Signaling In Immune System Pathway (366171); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Extracellular Matrix Organization Pathway (576262); Glucocorticoid Receptor Regulatory Network Pathway (138014)

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]

ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta. Protein type: Cell adhesion; Membrane protein, integral; Immunoglobulin superfamily. Chromosomal Location of Human Ortholog: 19p13.3-p13.2. Cellular Component: extracellular space; focal adhesion; cell surface; membrane; integral to plasma membrane; plasma membrane; immunological synapse; external side of plasma membrane. Molecular Function: integrin binding; viral receptor activity; protein binding; transmembrane receptor activity; receptor activity. Biological Process: extracellular matrix organization and biogenesis; entry of virus into host cell; positive regulation of nitric oxide biosynthetic process; T cell antigen processing and presentation; response to organic cyclic substance; activation of NF-kappaB transcription factor; positive regulation of cellular extravasation; regulation of cell shape; leukocyte adhesion; cellular response to nutrient levels; sensory perception of sound; ovarian follicle development; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; membrane to membrane docking; response to sulfur dioxide; cell adhesion; regulation of leukocyte mediated cytotoxicity; acute inflammatory response to antigenic stimulus; regulation of cell adhesion; response to drug; virion attachment, binding of host cell surface receptor; negative regulation of calcium ion transport; regulation of immune response; cytokine and chemokine mediated signaling pathway; response to amphetamine; cell aging; response to amino acid stimulus; heterophilic cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; positive regulation of actin filament polymerization; response to copper ion; positive regulation of vasoconstriction; adhesion to host; cell adhesion mediated by integrin; response to ionizing radiation; leukocyte migration. Disease: Malaria, Susceptibility To

96 Wells

455 USD