ELISA/assay

Human BMP-2 PicoKine ELISA Kit

MBS175918

1 x96 Wells

455 USD

ELISA Kit

Human BMP-2 PicoKine ELISA Kit

BMP-2

Bone morphogenetic protein 2; bone morphogenetic protein 2; BDA2; BMP-2; BMP-2A; Bmp2; BMP2_HUMAN; BMP2A; Bone morphogenetic protein 2; Bone morphogenetic protein 2A

Bone morphogenetic protein 2; bone morphogenetic protein 2; BMP-2A; bone morphogenetic protein 2A; bone morphogenetic protein 2; Bone morphogenetic protein 2

BMP2

BMP2; BMP2; BDA2; BMP2A

C8C060_HUMAN

Human

396

Natural and recombinant human BMP-2

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: For quantitative detection of human BMP-2 in bone tissue, cell culture supernates and serum. Detection Range: 62.5pg/ml-4000pg/ml

Sensitivity: < 2pg/ml. Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Principle of the assay: human BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-2 has been precoated onto 96-well plates. Standards(CHO,Q283-R396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BMP-2 amount of sample captured in plate. Background: Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein inducesmedulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinicaltrials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant human BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

C8C060

44,702 Da

Adipogenesis Pathway 198832!!BDNF Signaling Pathway 712093!!BMP Signalling And Regulation Pathway 198910!!Basal Cell Carcinoma Pathway 83113!!Basal Cell Carcinoma Pathway 525!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Elastic Fibre Formation Pathway 730310!!Extracellular Matrix Organization Pathway 576262!!Heart Development Pathway 198802

The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq, Jul 2008]

1 x96 Wells

455 USD