Human Cathepsin D PicoKine ELISA Kit | MyBioSource MBS175914 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Cathepsin D PicoKine ELISA Kit

catalog :

MBS175914

quantity :

96 Wells

price :

485 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS175914

products type :

ELISA Kit

products full name :

Human Cathepsin D PicoKine ELISA Kit

products short name :

Cathepsin D

products name syn :

Cathepsin D; cathepsin D; CATD_HUMAN; Cathepsin D heavy chain; Ceroid lipofuscinosis neuronal 10; CLN10; CPSD; ctsd; Lysosomal aspartyl protease

other names :

Cathepsin D; Cathepsin D; cathepsin D; lysosomal aspartyl protease; lysosomal aspartyl peptidase; ceroid-lipofuscinosis, neuronal 10; epididymis secretory sperm binding protein Li 130P; cathepsin D

products gene name :

CTSD

other gene names :

CTSD; CTSD; CPSD; CLN10; HEL-S-130P; CPSD

uniprot entry name :

CATD_HUMAN

reactivity :

Human

sequence length :

412

specificity :

Natural and recombinant human Cathepsin D

storage stability :

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

other info1 :

Samples: Cell culture supernates, serum and plasma (heparin, EDTA). Assay Type: Sandwich. Detection Range: 156pg/ml-10,000pg/ml. Sensitivity: < 10pg/ml

other info2 :

Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

products description :

Principle of the assay: human Cathepsin D ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cathepsin D has been precoated onto 96-well plates. Standards(NSO, L21-L412) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cathepsin D amount of sample captured in plate. Background: Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.

ncbi gi num :

115717

ncbi acc num :

P07339.1

uniprot acc num :

P07339

ncbi mol weight :

44,552 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Ceramide Signaling Pathway (138023); Collagen Degradation Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Direct P53 Effectors Pathway (137939); Extracellular Matrix Organization Pathway (576262); Immune System Pathway (106386); LKB1 Signaling Events Pathway (137957); Lysosome Pathway (99052); Lysosome Pathway (96865)

ncbi summary :

This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. [provided by RefSeq, Jul 2008]

uniprot summary :

CTSD: Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. Consists of a light chain and a heavy chain. Belongs to the peptidase A1 family. Protein type: Autophagy; Motility/polarity/chemotaxis; Protease; EC 3.4.23.5. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: extracellular matrix; extracellular space; lysosomal lumen; lysosome; melanosome; extracellular region. Molecular Function: protein binding; aspartic-type endopeptidase activity. Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis. Disease: Ceroid Lipofuscinosis, Neuronal, 10

size1 :

96 Wells

price1 :

485 USD

more info or order :

MyBioSource product webpage