ELISA/assay

Human IFN gamma PicoKine ELISA Kit

MBS175910

96 Wells

305 USD

ELISA Kit

Human IFN gamma PicoKine ELISA Kit

IFN gamma

Interferon gamma; interferon, gamma; IFG; IFI; IFN gamma; IFN Immune; IFNG; Interferon gamma; Interferon Gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon

Interferon gamma; Interferon gamma; interferon gamma; IFN-gamma; immune interferon; interferon, gamma; Immune interferon

IFNG

IFNG; IFNG; IFG; IFI; IFN-gamma

IFNG_HUMAN

Human

166

Natural and recombinant human IFNgamma

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Samples: Cell culture supernates and serum. Assay Type: Sandwich. Detection Range: 15.6pg/ml-1000pg/ml. Sensitivity: < 2pg/ml

Principle of the assay: human IFNgamma ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IFNgamma has been precoated onto 96-well plates. Standards (E.coli,Q24-Q166) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IFNgamma is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IFNgamma amount of sample captured in plate. Background: Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts.1 IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8cytotoxic T cells, and is vital for the control of microbial pathogens.2 Interferon gamma is believed to be crucial forhost defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis.3 IFN-gamma activates human macrophage oxidative metabolism andantimicrobial activity.4 In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions.IFN-gamma plays an important role in the control of neointima proliferation.5

124479

P01579.1

P01579

19,348 Da

ATF-2 Transcription Factor Network Pathway (138006); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485)

This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009]

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family. Protein type: Membrane protein, integral; Secreted; Secreted, signal peptide; Cytokine. Chromosomal Location of Human Ortholog: 12q14. Cellular Component: extracellular space; extracellular region; external side of plasma membrane. Molecular Function: interferon-gamma receptor binding; cytokine activity. Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; positive regulation of osteoclast differentiation; negative regulation of smooth muscle cell proliferation; positive regulation of interleukin-12 production; positive regulation of interleukin-23 production; apoptosis; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; positive regulation of killing of cells of another organism; negative regulation of transcription from RNA polymerase II promoter; sensory perception of mechanical stimulus; positive regulation of interleukin-1 beta secretion; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; neutrophil chemotaxis; positive regulation of synaptic transmission, cholinergic; adaptive immune response; CD8-positive, alpha-beta T cell differentiation during immune response; negative regulation of myelination; unfolded protein response; response to virus; cytokine and chemokine mediated signaling pathway; positive regulation of tumor necrosis factor production; defense response to protozoan; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; negative regulation of interleukin-17 production; positive regulation of interleukin-12 biosynthetic process; positive regulation of chemokine biosynthetic process; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; cell motility; positive regulation of neuron differentiation; regulation of insulin secretion. Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Mycobacterium Tuberculosis, Susceptibility To; Tuberous Sclerosis 2; Human Immunodeficiency Virus Type 1, Susceptibility To

96 Wells

305 USD